{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T11:14:44Z","timestamp":1789902884777,"version":"4.0.1"},"reference-count":58,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2010,1,20]],"date-time":"2010-01-20T00:00:00Z","timestamp":1263945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2010,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cuprizone (bis\u2010cyclohexanone oxaldihydrazone) was previously shown to induce demyelination in white matter enriched brain structures. In the present study we used the cuprizone demyelination model in transgenic mice expressing the enhanced green fluorescent protein (GFP) under the 2\u2032\u20103\u2032\u2010cyclic nucleotide 3\u2032\u2010phosphodiesterase (CNPase) promoter. The use of these particular transgenic mice allows easy detection of cells belonging to the entire oligodendroglial (OLG) lineage, ranging from OLG precursors to mature myelinating OLGs. We were able to evaluate the precise extent of oligodendroglial cell damage and recovery within the murine adult central nervous system (CNS) after inducing demyelination by acute cuprizone intoxication. A generalized loss of GFP+ cells was observed after cuprizone exposure and correlated with a decline in myelin basic protein (MBP) expression. OLGs were depleted in many brain areas that were previously thought to be unaffected by cuprizone treatment. Thus, in addition to the well\u2010known cuprizone effects on the medial corpus callosum, we also found a loss of GFP+ cells in most brain structures, particularly in the caudatus putamen, cortex, anterior commissure, olfactory bulb, hippocampus, optic chiasm, brainstem, and cingulum. Loss of GFP+ cells was accompanied by extensive astrogliosis and microglial activation, although neurons were not affected. Interestingly, cuprizone\u2010treated animals showed both activation of GFAP expression and a higher proliferation rate in subventricular zone cells. A week after cuprizone removal from the diet, GFP+ oligodendroglial cells began repopulating the damaged structures. GFP expression precedes that of MBP and allows OLG detection before myelin restoration. J. Comp. Neurol. 518:2261\u20132283, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.22330","type":"journal-article","created":{"date-parts":[[2010,1,20]],"date-time":"2010-01-20T22:37:40Z","timestamp":1264027060000},"page":"2261-2283","source":"Crossref","is-referenced-by-count":31,"title":["Cuprizone\u2010induced demyelination in CNP::GFP transgenic mice"],"prefix":"10.1002","volume":"518","author":[{"given":"Lucas","family":"Silvestroff","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Bartucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Soto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vittorio","family":"Gallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juana","family":"Pasquini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Franco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,4,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2005.12.027"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1938"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.jnen.0000205142.08716.7e"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19960901)45:5<558::AID-JNR6>3.0.CO;2-B"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000048712"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(73)90119-6"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/249577a0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2009.01219.x"},{"key":"e_1_2_6_10_1","first-page":"95","article-title":"Expression of Myelin basic protein genes in the developing mouse brain","volume":"12","author":"Campagnoni A","year":"1989","journal-title":"Dev Neurobiol"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10145"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.2005.00656.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80359-1"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4057-07.2007"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-5-44"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2003.09.011"},{"key":"e_1_2_6_17_1","doi-asserted-by":"crossref","first-page":"1866","DOI":"10.1523\/JNEUROSCI.10-06-01866.1990","article-title":"Cerebral white matter contains PDGF\u2010responsive precursor to O2A cells","volume":"10","author":"Grinspan JB","year":"1990","journal-title":"J Neurosci"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(98)00168-4"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21238"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.06.012"},{"key":"e_1_2_6_21_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.braindev.2008.06.005","article-title":"Basic fibroblast growth factor stimulates the proliferation and differentiation of neural stem cells in neonatal rats after ischemic brain injury","volume":"31","author":"Jin\u2010qiao S","year":"2008","journal-title":"Brain Dev"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00310.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2002.00954.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1179\/014788807794775675","article-title":"Histochemistry of staining methods for normal and degenerating myelin in the central and peripheral nervous system","volume":"30","author":"Kiernan JA","year":"2007","journal-title":"J Histotechnol"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21917"},{"key":"e_1_2_6_26_1","first-page":"40","article-title":"Experimental demyelination caused by primary oligodendrocyte dystrophy. 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