{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:09:09Z","timestamp":1791050949366,"version":"4.1.0"},"reference-count":84,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,4,22]],"date-time":"2011-04-22T00:00:00Z","timestamp":1303430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2011,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The zinc finger transcription factor Gli3 is essential for normal development of the forebrain. Mutant mice with no functional Gli3 (<jats:italic>extra\u2010toes<\/jats:italic>,<jats:italic>Gli3<\/jats:italic><jats:sup><jats:italic>Xt\/Xt<\/jats:italic><\/jats:sup>mutants) display a massive reduction in the size of the telencephalic lobes and absence of dorsomedial telencephalic structures, including the cortical hem, which normally expresses a number of Wnt molecules essential for patterning the hippocampus. Dorsomedial telencephalic Wnt activity, transduced through the Wnt\/\u03b2\u2010catenin signaling pathway, is also required for hippocampal specification and dorsoventral telencephalic patterning. Wnts whose normal expression is restricted to the cortical hem are completely absent in<jats:italic>Gli3<\/jats:italic><jats:sup><jats:italic>Xt\/Xt<\/jats:italic><\/jats:sup>embryos, but some expression of those Wnts with a broader expression domain persists, raising the possibility that Wnt\/\u03b2\u2010catenin signaling may still be active in this mutant. We examined whether the<jats:italic>Wnt<\/jats:italic>expression that persists in the<jats:italic>Gli3<\/jats:italic><jats:sup><jats:italic>Xt\/Xt<\/jats:italic><\/jats:sup>mutant neocortex activates Wnt\/\u03b2\u2010catenin signaling, using the BAT\u2010gal transgenic reporter. We found Wnt\/\u03b2\u2010catenin signaling consistently decreased in the forebrains of<jats:italic>Gli3<\/jats:italic><jats:sup><jats:italic>Xt\/Xt<\/jats:italic><\/jats:sup>mutants, even prior to the formation of the cortical hem. This is accompanied by a severe reduction in expression of<jats:italic>Wnt7b<\/jats:italic>and<jats:italic>Wnt8b<\/jats:italic>at the lateral edges of the anterior neural plate that will give rise to the pallium. In addition, we found a significant increase in the expression of rostroventral markers of the anterior neural plate that will give rise to the basal forebrain. Our data reveal that Gli3 is required at the neural plate stage to regulate<jats:italic>Wnt<\/jats:italic>expression and Wnt\/\u03b2\u2010catenin signaling in the presumptive forebrain and confirm its previously proposed role in patterning the anterior neural plate. J. Comp. Neurol. 519:1640\u20131657, 2011. \u00a9 2011 Wiley\u2010Liss, Inc<\/jats:p>","DOI":"10.1002\/cne.22592","type":"journal-article","created":{"date-parts":[[2011,1,12]],"date-time":"2011-01-12T17:51:20Z","timestamp":1294854680000},"page":"1640-1657","source":"Crossref","is-referenced-by-count":23,"title":["Wnt\/\u03b2\u2010catenin signaling is disrupted in the<i>extra\u2010toes<\/i>(<i>Gli3<\/i><sup><i>Xt\/Xt<\/i><\/sup>) mutant from early stages of forebrain development, concomitant with anterior neural plate patterning defects"],"prefix":"10.1002","volume":"519","author":[{"given":"Vassiliki","family":"Fotaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Price","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John 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