{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:18:47Z","timestamp":1760170727803},"reference-count":103,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2011,9,27]],"date-time":"2011-09-27T00:00:00Z","timestamp":1317081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previously, we compared molecular profiles of one population of wild\u2010type (WT) mouse facial motoneurons (FMNs) surviving with FMNs undergoing significant cell death after axotomy. Regardless of their ultimate fate, injured FMNs respond with a vigorous pro\u2010survival\/regenerative molecular response. In contrast, the neuropil surrounding the two different injured FMN populations contained distinct molecular differences that support a causative role for glial and\/or immune\u2010derived molecules in directing contrasting responses of the same cell types to the same injury. In the current investigation, we utilized the facial nerve axotomy model and a presymptomatic amyotrophic lateral sclerosis (ALS) mouse (SOD1) model to experimentally mimic the axonal die\u2010back process observed in ALS pathogenesis without the confounding variable of disease onset. Presymptomatic SOD1 mice had a significant decrease in FMN survival compared with WT, which suggests an increased susceptibility to axotomy. Laser microdissection was used to accurately collect uninjured and axotomized facial motor nuclei of WT and presymptomatic SOD1 mice for mRNA expression pattern analyses of pro\u2010survival\/pro\u2010regeneration genes, neuropil\u2010specific genes, and genes involved in or responsive to the interaction of FMNs and non\u2010neuronal cells. Axotomized presymptomatic SOD1 FMNs displayed a dynamic pro\u2010survival\/regenerative response to axotomy, similar to WT, despite increased cell death. However, significant differences were revealed when the axotomy\u2010induced gene expression response of presymptomatic SOD1 neuropil was compared with WT. We propose that the increased susceptibility of presymptomatic SOD1 FMNs to axotomy\u2010induced cell death and, by extrapolation, disease progression, is not intrinsic to the motoneuron, but rather involves a dysregulated response by non\u2010neuronal cells in the surrounding neuropil. J. Comp. Neurol. 519:3488\u20133506, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.22718","type":"journal-article","created":{"date-parts":[[2011,7,28]],"date-time":"2011-07-28T16:21:40Z","timestamp":1311870100000},"page":"3488-3506","source":"Crossref","is-referenced-by-count":11,"title":["Differential gene expression in the axotomized facial motor nucleus of presymptomatic SOD1 mice"],"prefix":"10.1002","volume":"519","author":[{"given":"Nichole A.","family":"Mesnard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia M.","family":"Sanders","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn J.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1784-04.2004"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(97)00093-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.57.7.1282"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023258923002"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10750"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200412030-00018"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.06.085"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.wnr.0000199465.54907.74"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2007.09.084"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607423103"},{"key":"e_1_2_6_12_1","first-page":"73","article-title":"Immunohistochemical localization of TNFalpha and its receptors in the rodent central nervous system","volume":"98","author":"Bigini P","year":"2004","journal-title":"Methods Mol Med"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(72)90398-8"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"4333","DOI":"10.1523\/JNEUROSCI.5276-03.2004","article-title":"CD4\u2010positive T cell\u2010mediated neuroprotection requires dual compartment antigen presentation","volume":"24","author":"Byram SC","year":"2004","journal-title":"J Neurosci"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2006.08.006"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009802"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-15-j0004.2000"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90339-A"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0702372"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8351519"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2008.09.002"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.2000.0318"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2009.03.007"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(01)00242-X"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2007.0466"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2003.10.004"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra022366"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02740621"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-7-8"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01674208"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(88)90585-X"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8209258"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(02)01140-0"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10161"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.18.10896"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2007.07.003"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-9961(03)00087-1"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.09-03-00893.1989"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004010100449"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(85)90212-3"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-06-03620.1994"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022071123255"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018596316465"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022019106417"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(19990915)40:4<560::AID-NEU11>3.0.CO;2-I"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19990915)57:6<817::AID-JNR6>3.0.CO;2-Q"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007157105835"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2004.05.004"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199805110-00028"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199605310-00021"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-10-03622.2000"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1996.tb01588.x"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(96)10049-7"},{"key":"e_1_2_6_54_1","first-page":"103","article-title":"Principles of neuronal regeneration","volume":"66","author":"Kreutzberg GW","year":"1996","journal-title":"Acta Neurochir Suppl"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00179953"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(08)60183-X"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)01034-X"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00448-7"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2002.7908"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0911-05.2005"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2010.05.019"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.1.45"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22156"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2003.11.004"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(20000103)416:1<112::AID-CNE9>3.0.CO;2-K"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(95)90042-X"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(94)90122-8"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410310605"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2008.04.001"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)01177-0"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-10-03369.2001"},{"key":"e_1_2_6_72_1","first-page":"87","article-title":"Does the pathological factor in amyotrophic lateral sclerosis (ALS) damage also astrocytes?","volume":"36","author":"Rafalowska J","year":"1998","journal-title":"Folia Neuropathol"},{"key":"e_1_2_6_73_1","article-title":"Is the spinal cord motoneuron exclusively a target in ALS? Comparison between astroglial reactivity in a rat model of familial ALS and in human sporadic ALS cases","author":"Rafalowska J","year":"2009","journal-title":"Neurol Res."},{"key":"e_1_2_6_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2000.tb00268.x"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/362059a0"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200105313442207"},{"key":"e_1_2_6_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22424"},{"key":"e_1_2_6_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2007.02.028"},{"key":"e_1_2_6_79_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20620"},{"key":"e_1_2_6_80_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007048903862"},{"key":"e_1_2_6_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(07)96002-2"},{"key":"e_1_2_6_82_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-11-j0004.1999"},{"key":"e_1_2_6_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-1591(03)00028-X"},{"key":"e_1_2_6_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2004.07.005"},{"key":"e_1_2_6_85_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.expneurol.2009.04.031","article-title":"Electrical stimulation and testosterone differentially enhance expression of regeneration\u2010associated genes","volume":"223","author":"Sharma N","year":"2009","journal-title":"Exp Neurol"},{"key":"e_1_2_6_86_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1789.2001.00361.x"},{"key":"e_1_2_6_87_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1317.080"},{"key":"e_1_2_6_88_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.12.030189.001015"},{"key":"e_1_2_6_89_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-08-02528.1991"},{"key":"e_1_2_6_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2003.08.007"},{"key":"e_1_2_6_91_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2004.03593.x"},{"key":"e_1_2_6_92_1","doi-asserted-by":"crossref","first-page":"00024","DOI":"10.1042\/AN20090017","article-title":"Exacerbation of facial motoneuron loss after facial nerve axotomy in CCR3\u2010deficient mice","volume":"1","author":"Wainwright DA","year":"2009","journal-title":"ASN Neuro"},{"key":"e_1_2_6_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2009.05.002"},{"key":"e_1_2_6_94_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)01351-1"},{"key":"e_1_2_6_95_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-10-03457.2001"},{"key":"e_1_2_6_96_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq106"},{"key":"e_1_2_6_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2007.10.006"},{"key":"e_1_2_6_98_1","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/j.bbi.2010.08.004","article-title":"IL\u201010 within the CNS is necessary for CD4(+) T cells to mediate neuroprotection","volume":"25","author":"Xin J","year":"2010","journal-title":"Brain Behav Immun"},{"key":"e_1_2_6_99_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802556105"},{"key":"e_1_2_6_100_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-3042.2001.00683.x"},{"key":"e_1_2_6_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(02)00944-8"},{"key":"e_1_2_6_102_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20379"},{"key":"e_1_2_6_103_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2006.11.003"},{"key":"e_1_2_6_104_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20676"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.22718","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.22718","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T13:56:53Z","timestamp":1700575013000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.22718"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,27]]},"references-count":103,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["10.1002\/cne.22718"],"URL":"https:\/\/doi.org\/10.1002\/cne.22718","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,27]]}}}