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We have shown that the fish ortholog of the phospholipase C gene (<jats:italic>plc\u2010\u03b22<\/jats:italic>) is expressed in a subpopulation of taste bud cells that transmit taste stimuli to the central nervous system to evoke favorable and aversive behaviors. We generated transgenic medaka expressing wheat germ agglutinin (WGA) under the control of a regulatory region of the medaka <jats:italic>plc\u2010\u03b22<\/jats:italic> gene to analyze the neuronal circuit connected to these sensory cells. Immunohistochemical analysis of the transgenic fish 12 days post fertilization revealed that the WGA protein was transferred to cranial sensory ganglia and several nuclei in the hindbrain. WGA signals were also detected in the secondary gustatory nucleus in the hindbrain of 3\u2010month\u2010old transgenic fish. WGA signals were observed in several diencephalic and telencephalic regions in 9\u2010month\u2010old transgenic fish. The age\u2010dependent increase in the labeled brain regions strongly suggests that labeling occurred at taste bud cells and progressively extended to cranial nerves and neurons in the central nervous system. These data are the first to demonstrate the tracing of higher order gustatory neuronal circuitry that is associated with a specific subpopulation of taste bud cells. These results provide insight into the basic neuronal architecture of gustatory information processing that is common among vertebrates. J. Comp. Neurol. 521:1781\u20131802, 2013. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cne.23256","type":"journal-article","created":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T14:06:17Z","timestamp":1351778777000},"page":"1781-1802","source":"Crossref","is-referenced-by-count":9,"title":["Transgenic labeling of higher order neuronal circuits linked to phospholipase C\u2010\u03b22\u2013expressing taste bud cells in medaka 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