{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:00:45Z","timestamp":1791255645955,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,8,30]],"date-time":"2014-08-30T00:00:00Z","timestamp":1409356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["MH093667"],"award-info":[{"award-number":["MH093667"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["MH090487"],"award-info":[{"award-number":["MH090487"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The lateral habenula (LHb) is part of the habenula complex of the dorsal thalamus. Recent studies of the LHb have focused on its projections to the ventral tegmental area (VTA) and rostromedial tegmental nucleus (RMTg), which contain \u03b3\u2010aminobutyric acid (GABA)ergic neurons that mediate reward prediction error via inhibition of dopaminergic activity. However, older studies in the rat have also identified LHb outputs to the lateral and posterior hypothalamus, median raphe, dorsal raphe, and dorsal tegmentum. Although these studies have shown that the medial and lateral divisions of the LHb have somewhat distinct projections, the topographic specificity of LHb efferents is not completely understood, and the relative extent of these projections to brainstem targets is unknown. Here we have used anterograde tracing with adeno\u2010associated virus\u2013mediated expression of green fluorescent protein, combined with serial two\u2010photon tomography, to map the efferents of the LHb on a standard coordinate system for the entire mouse brain, and reconstruct the efferent pathways of the LHb in three dimensions. Using automated quantitation of fiber density, we show that in addition to the RMTg, the median raphe, caudal dorsal raphe, and pontine central gray are major recipients of LHb efferents. By using retrograde tract tracing with cholera toxin subunit B, we show that LHb neurons projecting to the hypothalamus, VTA, median raphe, caudal dorsal raphe, and pontine central gray reside in characteristic, but sometimes overlapping regions of the LHb. Together these results provide the anatomical basis for systematic studies of LHb function in neural circuits and behavior in mice. J. Comp. Neurol. 523:32\u201360, 2015. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cne.23662","type":"journal-article","created":{"date-parts":[[2014,8,7]],"date-time":"2014-08-07T07:09:05Z","timestamp":1407395345000},"page":"32-60","source":"Crossref","is-referenced-by-count":153,"title":["Efferent Pathways of the Mouse Lateral Habenula"],"prefix":"10.1002","volume":"523","author":[{"given":"Lely A.","family":"Quina","sequence":"first","affiliation":[{"name":"Center for Integrative Brain Research Seattle Children's Research Institute Seattle Washington 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynne","family":"Tempest","sequence":"additional","affiliation":[{"name":"Center for Integrative Brain Research Seattle Children's Research Institute Seattle Washington 98101"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Ng","sequence":"additional","affiliation":[{"name":"Allen Institute for Brain Science Seattle Washington 98103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie A.","family":"Harris","sequence":"additional","affiliation":[{"name":"Allen Institute for Brain Science Seattle Washington 98103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Ferguson","sequence":"additional","affiliation":[{"name":"Center for Integrative Brain Research Seattle Children's Research Institute Seattle Washington 98101"},{"name":"Department of Psychiatry and Behavioral Sciences University of Washington Seattle Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Jhou","sequence":"additional","affiliation":[{"name":"Department of Neurosciences Medical University of South Carolina Charleston South Carolina 29425"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric E.","family":"Turner","sequence":"additional","affiliation":[{"name":"Center for Integrative Brain Research Seattle Children's Research Institute Seattle Washington 98101"},{"name":"Department of Neurosciences Medical University of South Carolina Charleston South Carolina 29425"},{"name":"Center of Human Development and Disability University of Washington Seattle Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(77)90291-8"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4369-12.2013"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02934-1"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19990428)407:1<130::AID-CNE10>3.0.CO;2-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)91410-2"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23080"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2007.05738.x"},{"key":"e_1_2_9_9_1","volume-title":"Allen reference atlas: a digital color brain atlas of the C57Black\/6J male mouse","author":"Dong HW","year":"2008"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(95)00435-1"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10566"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0012-07.2007"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1301650"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22787"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-012-9827-1"},{"key":"e_1_2_9_16_1","first-page":"21","article-title":"Adeno\u2010associated viral vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice","author":"Harris JA","year":"2012","journal-title":"Curr Protoc Neurosci"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901870103"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1384-11.2011"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2927-13.2013"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.02.001"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21891"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3634-12.2013"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21983"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90042-M"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21951"},{"key":"e_1_2_9_26_1","first-page":"RA261","article-title":"Habenular and interpeduncularis nuclei: shared components in multiple\u2010function networks","volume":"10","author":"Klemm WR","year":"2004","journal-title":"Med Sci Monit"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11527"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-153"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09742"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90131-T"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3078"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05860"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2233"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13186"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2009.06924.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(00)00194-7"},{"key":"e_1_2_9_37_1","volume-title":"The rat brain in stereotaxic coordinates","author":"Paxinos G","year":"2005"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2005.04110.x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2837-05.2005"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2430-09.2009"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1854"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.12.038"},{"key":"e_1_2_9_43_1","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1002\/cne.23533","article-title":"Lateral habenula and the rostromedial tegmental nucleus innervate neurochemically distinct subdivisions of the dorsal raphe nucleus in the rat","volume":"522","author":"C Sego","year":"2014","journal-title":"J Comp Neurol"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3604-09.2010"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3145"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0149-7634(82)90003-3"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10905"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23554"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.10.047"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2010.07.034"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.23662","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.23662","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T07:17:41Z","timestamp":1696576661000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.23662"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,30]]},"references-count":49,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1002\/cne.23662"],"URL":"https:\/\/doi.org\/10.1002\/cne.23662","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,30]]}}}