{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:34:19Z","timestamp":1790307259770,"version":"4.1.0"},"reference-count":86,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2015,8,11]],"date-time":"2015-08-11T00:00:00Z","timestamp":1439251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FWF","award":["P21092"],"award-info":[{"award-number":["P21092"]}]},{"name":"FWF","award":["I914"],"award-info":[{"award-number":["I914"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2015,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The development of the mammalian brain requires the generation, migration, and differentiation of neurons, cellular processes that are dependent on a dynamic microtubule cytoskeleton. Mutations in tubulin genes, which encode for the structural subunits of microtubules, cause detrimental neurological disorders known as the <jats:italic>tubulinopathies<\/jats:italic>. The disease spectra associated with different tubulin genes are overlapping but distinct, an observation believed to reflect functional specification of this multigene family. Perturbation of the \u03b2\u2010tubulin <jats:italic>TUBB2B<\/jats:italic> is known to cause polymicrogyria, pachygyria, microcephaly, and axon guidance defects. Here we provide a detailed analysis of the expression pattern of its murine homolog <jats:italic>Tubb2b<\/jats:italic>. The generation and characterization of BAC\u2010transgenic eGFP reporter mouse lines has revealed that it is highly expressed in progenitors and postmitotic neurons during cortical development. This contrasts with the 8\u2010week\u2010old cortex, in which <jats:italic>Tubb2b<\/jats:italic> expression is restricted to macroglia, and expression is almost completely absent in mature neurons. This developmental transition in neurons is mirrored in the adult hippocampus and the cerebellum but is not a universal feature of <jats:italic>Tubb2b<\/jats:italic>; its expression persists in a population of postmitotic neurons in the 8\u2010week\u2010old retina. We propose that the dynamic spatial and temporal expression of Tubb2b reflects specific functional requirements of the microtubule cytoskeleton. J. Comp. Neurol. 523:2161\u20132186, 2015. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cne.23836","type":"journal-article","created":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T23:35:14Z","timestamp":1435102514000},"page":"2161-2186","source":"Crossref","is-referenced-by-count":40,"title":["The expression of <i>tubb2b<\/i> undergoes a developmental transition in murine cortical neurons"],"prefix":"10.1002","volume":"523","author":[{"given":"Martin","family":"Breuss","sequence":"first","affiliation":[{"name":"IMP\u2014Research Institute of Molecular Pathology Vienna Biocenter (VBC), Vienna 1030 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmin","family":"Morandell","sequence":"additional","affiliation":[{"name":"IMP\u2014Research Institute of Molecular Pathology Vienna Biocenter (VBC), Vienna 1030 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Nimpf","sequence":"additional","affiliation":[{"name":"IMP\u2014Research Institute of Molecular Pathology Vienna Biocenter (VBC), Vienna 1030 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Gstrein","sequence":"additional","affiliation":[{"name":"IMP\u2014Research Institute of Molecular Pathology Vienna Biocenter (VBC), Vienna 1030 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattias","family":"Lauwers","sequence":"additional","affiliation":[{"name":"IMP\u2014Research Institute of Molecular Pathology Vienna Biocenter (VBC), Vienna 1030 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Hochstoeger","sequence":"additional","affiliation":[{"name":"IMP\u2014Research Institute of Molecular Pathology Vienna Biocenter (VBC), Vienna 1030 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Braun","sequence":"additional","affiliation":[{"name":"IMP\u2014Research Institute of Molecular Pathology Vienna Biocenter (VBC), Vienna 1030 Austria"},{"name":"Centre for Genomic Regulation (CRG) 08003 Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelvin","family":"Chan","sequence":"additional","affiliation":[{"name":"Medical Scientist Training Program Stony Brook University Medical Center Stony Brook New York 11794"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edmundo R.","family":"S\u00e1nchez Guajardo","sequence":"additional","affiliation":[{"name":"Advanced Microscopy Campus Science Support Facilities (CSF) Vienna 1030 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Advanced Microscopy Campus Science Support Facilities (CSF) Vienna 1030 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Suplata","sequence":"additional","affiliation":[{"name":"Rudolf Virchow Center DFG\u2010Research Center for Experimental Biomedicine of the University of W\u00fcrzburg W\u00fcrzburg 97080 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin G.","family":"Heinze","sequence":"additional","affiliation":[{"name":"Rudolf Virchow Center DFG\u2010Research Center for Experimental Biomedicine of the University of W\u00fcrzburg W\u00fcrzburg 97080 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kareem","family":"Elsayad","sequence":"additional","affiliation":[{"name":"Advanced Microscopy Campus Science Support Facilities (CSF) Vienna 1030 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Keays","sequence":"additional","affiliation":[{"name":"IMP\u2014Research Institute of Molecular Pathology Vienna Biocenter (VBC), Vienna 1030 Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1824"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002388"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.12.021"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awu082"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19961007)374:1<52::AID-CNE4>3.0.CO;2-M"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-008-9128-9"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21646"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2010.03.019"},{"key":"e_1_2_8_10_1","first-page":"75","volume-title":"Microtubules and neurodevelopmental disease: the movers and the makers. cellular and molecular control of neuronal migration","author":"Breuss M","year":"2014"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.11.017"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb03074.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/39.5.1707903"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds393"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.3.381"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90286-6"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90238-X"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.8.4877"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aws338"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00633-0"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2008.03094.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2013.71"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2010.10.002"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.090571"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.12.022"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.3.395"},{"key":"e_1_2_8_27_1","first-page":"987","volume-title":"Cell motility","author":"Fulton C","year":"1976"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5457.1433"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(00)01607-6"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-12-07319.1994"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19990301)405:1<45::AID-CNE4>3.0.CO;2-M"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1739"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2928"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20777"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004120050256"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.3.1009"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.3.481"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.380"},{"key":"e_1_2_8_39_1","first-page":"315","volume-title":"Tubulin\u2010related malformations of cortical development. Cytoskeleton Hum Dis","author":"Jaglin X","year":"2012"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3227"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-007-9034-4"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1159\/000319663"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-0436.2003.710301.x"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.052969"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2012.03675.x"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2011.05.004"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-06-162610"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.3.852"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(97)01168-9"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20325"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.085852"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22489"},{"key":"e_1_2_8_53_1","doi-asserted-by":"crossref","first-page":"2723","DOI":"10.1242\/jcs.114.15.2723","article-title":"The extended tubulin superfamily","volume":"114","author":"McKean PG","year":"2001","journal-title":"J Cell Sci"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.04.019"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.116.1.201"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.2.225"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21037"},{"key":"e_1_2_8_58_1","article-title":"TUBB5 and its disease\u2010associated mutations influence the terminal differentiation and dendritic spine densities of cerebral cortical neurons","author":"Ngo L","year":"2014","journal-title":"Hum Mol Genet ddu238"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052396"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09146"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq377"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.05.042"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.033498"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8749.2012.04316.x"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09611"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt538"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.3.6.870"},{"key":"e_1_2_8_68_1","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1242\/jcs.107.8.2165","article-title":"Mutations in the Caenorhabditis elegans beta\u2010tubulin gene mec\u20107: effects on microtubule assembly and stability and on tubulin autoregulation","volume":"107","author":"Savage C","year":"1994","journal-title":"J Cell Sci"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2920"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt255"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.09.002"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.11.018"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.138.4.821"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.12.011"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2011.01.003"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.04.013"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-7-research0034"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhu125"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2579-12.2013"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.6.7.2409"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-007-0432-4"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1109\/TIP.2008.917103"},{"key":"e_1_2_8_83_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.5.1903"},{"key":"e_1_2_8_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.10.003"},{"key":"e_1_2_8_85_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13287"},{"key":"e_1_2_8_86_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.096776"},{"key":"e_1_2_8_87_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2012.260"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.23836","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.23836","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T22:26:34Z","timestamp":1696631194000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.23836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,11]]},"references-count":86,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2015,10,15]]}},"alternative-id":["10.1002\/cne.23836"],"URL":"https:\/\/doi.org\/10.1002\/cne.23836","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,11]]}}}