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We employed a double\u2010label method to map the ascending projections of pruriceptive and nociceptive trigeminal and spinal neurons. The retrograde tracer fluorogold (FG) was stereotaxically injected into the right thalamus or lateral parabrachial area (LPb) in mice. Seven days later, mice received intradermal (id) microinjection of histamine, chloroquine, capsaicin, or vehicle into the left cheek. Histamine, chloroquine, and capsaicin intradermally elicited similar distributions of Fos\u2010positive neurons in the medial aspect of the superficial medullary and spinal dorsal horn from the trigeminal subnucleus caudalis to C2. Among neurons retrogradely labeled from the thalamus, 43%, 8%, and 22% were Fos\u2010positive following id histamine, chloroquine, or capsaicin. Among the Fos\u2010positive neurons following pruritic or capsaicin stimuli, \u223c1\u20132% were retrogradely labeled with FG. Trigeminoparabrachial projection neurons exhibited a higher incidence of double labeling in the superficial dorsal horn. Among the neurons retrogradely labeled from LPb, 36%, 29%, and 33% were Fos positive following id injection of histamine, chloroquine, and capsaicin, respectively. Among Fos\u2010positive neurons elicited by id histamine, chloroquine, and capsaicin, respectively, 3.7%, 4.3%, and 4.1% were retrogradely labeled from LPb. The present results indicate that, overall, relatively small subpopulations of pruriceptive and\/or nociceptive neurons innervating the cheek project to thalamus or LPb. These results imply that the vast majority of pruritogen\u2010 and algogen\u2010responsive spinal neurons are likely to function as interneurons relaying information to projection neurons and\/or participating in segmental nocifensive circuits. J. Comp. Neurol. 524:244\u2013256, 2016. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cne.23839","type":"journal-article","created":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T08:22:25Z","timestamp":1435047745000},"page":"244-256","source":"Crossref","is-referenced-by-count":31,"title":["Anatomical evidence of pruriceptive trigeminothalamic and trigeminoparabrachial projection neurons in mice"],"prefix":"10.1002","volume":"524","author":[{"given":"Tasuku","family":"Akiyama","sequence":"first","affiliation":[{"name":"Temple Itch Center Department of Dermatology Department of Anatomy and Cell Biology Temple University School of Medicine Philadelphia Pennsylvania 19140"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Curtis","sequence":"additional","affiliation":[{"name":"Department of Neurobiology Physiology and Behavior University of California, Davis Davis California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Neurobiology Physiology and Behavior University of California, Davis Davis California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirela Iodi","family":"Carstens","sequence":"additional","affiliation":[{"name":"Department of Neurobiology Physiology and Behavior University of California, Davis Davis California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Carstens","sequence":"additional","affiliation":[{"name":"Department of Neurobiology Physiology and Behavior University of California, Davis Davis California 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