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Because of its preferential expression in nerve fibers, it has often been difficult to trace and identify the endogenous sources of the peptide in specific populations of neurons. Here, we introduce a transgenic mouse line that harbors in its genome a bacterial artificial chromosome containing an enhanced green fluorescent protein (EGFP) expression cassette inserted upstream of the PACAP ATG translation initiation codon. Analysis of expression in brain sections of these mice using a GFP antibody reveals EGFP expression in distinct neuronal perikarya and dendritic arbors in several major brain regions previously reported to express PACAP from using a variety of approaches, including radioimmunoassay, in situ hybridization, and immunohistochemistry with and without colchicine. EGFP expression in neuronal perikarya was modulated in a manner similar to PACAP gene expression in motor neurons after peripheral axotomy in the ipsilateral facial motor nucleus in the brainstem, providing an example in which the transgene undergoes proper regulation in vivo. These mice and the high\u2010resolution map obtained are expected to be useful in understanding the anatomical patterns of PACAP expression and its plasticity in the mouse. J. Comp. Neurol. 524:3827\u20133848, 2016. \u00a9 2016 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/cne.24035","type":"journal-article","created":{"date-parts":[[2016,5,19]],"date-time":"2016-05-19T23:27:08Z","timestamp":1463700428000},"page":"3827-3848","source":"Crossref","is-referenced-by-count":46,"title":["High\u2010resolution characterization of a PACAP\u2010EGFP transgenic mouse model for mapping PACAP\u2010expressing neurons"],"prefix":"10.1002","volume":"524","author":[{"given":"Michael C.","family":"Condro","sequence":"first","affiliation":[{"name":"Department of Psychiatry University of California Los Angeles, Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Matynia","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine University of California Los Angeles, Los Angeles California 90095"},{"name":"Brain Research Institute University of California Los Angeles, Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas N.","family":"Foster","sequence":"additional","affiliation":[{"name":"Institute of Neuro Imaging and Informatics, Keck School of Medicine University of Southern California Los Angeles California 90089"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukio","family":"Ago","sequence":"additional","affiliation":[{"name":"Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences Osaka University Osaka Japan 565\u20100871"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abha K.","family":"Rajbhandari","sequence":"additional","affiliation":[{"name":"Department of Psychiatry University of California Los Angeles, Los Angeles California 90095"},{"name":"Department of Psychology University of California Los Angeles, Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Van","sequence":"additional","affiliation":[{"name":"Molecular Biology Interdepartmental Program University of California, Los Angeles, Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhavaani","family":"Jayaram","sequence":"additional","affiliation":[{"name":"Department of Psychiatry University of California Los Angeles, Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachin","family":"Parikh","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine University of California Los Angeles, Los Angeles California 90095"},{"name":"Brain Research Institute University of California Los Angeles, Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna L.","family":"Diep","sequence":"additional","affiliation":[{"name":"Department of Psychiatry University of California Los Angeles, Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eileen","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Jules Stein Eye Institute, David Geffen School of Medicine University of California Los Angeles, Los Angeles California 90095"},{"name":"Brain Research Institute University of California Los Angeles, Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"May","sequence":"additional","affiliation":[{"name":"Department of Neurological Sciences University of Vermont Burlington Vermont 05405"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Wei","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Neuro Imaging and Informatics, Keck School of Medicine University of Southern California Los Angeles California 90089"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Waschek","sequence":"additional","affiliation":[{"name":"Department of Psychiatry University of California Los Angeles, Los Angeles California 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-129-5-2787"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.06.085"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.wnr.0000199465.54907.74"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112243"},{"key":"e_1_2_9_6_1","first-page":"51","article-title":"The mouse olfactory peduncle. 2. 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