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Unbeknownst to most researchers, however, <jats:italic>Pomc<\/jats:italic> in situ hybridization studies in the rat show specific labeling in the ventral wall of the hypothalamic third ventricle, which is formed by specialized ependymal cells, called tanycytes. Here we characterized this non\u2010neuronal POMC expression in detail using in situ hybridization and immunohistochemical techniques, and report two unique characteristics. First, POMC mRNA and precursor protein expression in non\u2010neuronal cells varies to a great degree as to the extent and abundance of expression. In brains with low\u2010level expression, POMC mRNA and protein was largely confined to a population of tanycytes within the infundibular stalk\/caudal median eminence, termed here \u03b3 tanycytes, and a subset of closely located \u03b2 and \u03b12 tanycytes. In brains with high\u2010level expression, POMC mRNA and protein was observed in the vast majority of \u03b12, \u03b2, and \u03b3 tanycytes. This variability was observed in both adult males and females; of 41 rats between 8 and 15 weeks of age, 17 had low\u2010, 9 intermediate\u2010, and 15 high\u2010level POMC expression in tanycytes. Second, unlike other known POMC\u2010expressing cells, tanycytes rarely contained detectable levels of adrenocorticotropin or \u03b1\u2010melanocyte\u2013stimulating hormone. The results indicate either a dynamic spatiotemporal pattern whereby low and high POMC syntheses in tanycytes occur periodically in each brain, or marked interindividual differences that may persist throughout adulthood. Future studies are required to examine these possibilities and elucidate the physiologic importance of POMC in tanycytes. J. Comp. 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