{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:53:12Z","timestamp":1791985992328,"version":"4.4.0"},"reference-count":62,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,10,6]],"date-time":"2016-10-06T00:00:00Z","timestamp":1475712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the South African Medical Research Council (MRC)","award":["415661"],"award-info":[{"award-number":["415661"]}]},{"name":"the South African National Research Foundation (NRF)","award":["UID93197"],"award-info":[{"award-number":["UID93197"]}]},{"name":"the University of Cape Town Research Council (URC) (to D.M.L.)"},{"name":"the Spanish Ministry of Education","award":["Research Project BFU2007-67139"],"award-info":[{"award-number":["Research Project BFU2007-67139"]}]},{"name":"the Regional Canary Islands Government","award":["ACIISI, Research Project SolSubC200801000281 and ULPAPD-08\/01-4 (to M.M.-M.)"],"award-info":[{"award-number":["ACIISI, Research Project SolSubC200801000281 and ULPAPD-08\/01-4 (to M.M.-M.)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The myelin\u2010associated protein Nogo\u2010A contributes to the failure of axon regeneration in the mammalian central nervous system (CNS). Inhibition of axon growth by Nogo\u2010A is mediated by the Nogo\u201066 receptor (NgR). Nonmammalian vertebrates, however, are capable of spontaneous CNS axon regeneration, and we have shown that retinal ganglion cell (RGC) axons regenerate in the lizard <jats:italic>Gallotia galloti<\/jats:italic>. Using immunohistochemistry, we observed spatiotemporal regulation of Nogo\u2010A and NgR in cell bodies and axons of RGCs during ontogeny. In the adult lizard, expression of Nogo\u2010A was associated with myelinated axon tracts and upregulated in oligodendrocytes during RGC axon regeneration. NgR became upregulated in RGCs following optic nerve injury. In in vitro studies, Nogo\u2010A\u2010Fc failed to inhibit growth of lizard RGC axons. The inhibitor of protein kinase A (pkA) activity KT5720 blocked growth of lizard RGC axons on substrates of Nogo\u2010A\u2010Fc, but not laminin. On patterned substrates of Nogo\u2010A\u2010Fc, KT5720 caused restriction of axon growth to areas devoid of Nogo\u2010A\u2010Fc. Levels of cyclic adenosine monophosphate (cAMP) were elevated over sustained periods in lizard RGCs following optic nerve lesion. We conclude that Nogo\u2010A and NgR are expressed in a mammalian\u2010like pattern and are upregulated following optic nerve injury, but the presence of Nogo\u2010A does not inhibit RGC axon regeneration in the lizard visual pathway. The results of outgrowth assays suggest that outgrowth\u2010promoting substrates and activation of the cAMP\/pkA signaling pathway play a key role in spontaneous lizard retinal axon regeneration in the presence of Nogo\u2010A. Restriction of axon growth by patterned Nogo\u2010A\u2010Fc substrates suggests that Nogo\u2010A may contribute to axon guidance in the lizard visual system. J. Comp. Neurol. 525:936\u2013954, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cne.24112","type":"journal-article","created":{"date-parts":[[2016,9,12]],"date-time":"2016-09-12T08:57:10Z","timestamp":1473670630000},"page":"936-954","source":"Crossref","is-referenced-by-count":6,"title":["Nogo\u2010A does not inhibit retinal axon regeneration in the lizard <i>Gallotia galloti<\/i>"],"prefix":"10.1002","volume":"525","author":[{"given":"Dirk M.","family":"Lang","sequence":"first","affiliation":[{"name":"Division of Physiological Sciences, Department of Human Biology University of Cape Town Observatory 7925 South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria del Mar","family":"Romero\u2010Alem\u00e1n","sequence":"additional","affiliation":[{"name":"Research Institute of Biomedical and Health Sciences University of Las Palmas de Gran Canaria 35016 Las Palmas Canary Islands Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryony","family":"Dobson","sequence":"additional","affiliation":[{"name":"Division of Physiological Sciences, Department of Human Biology University of Cape Town Observatory 7925 South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Santos","sequence":"additional","affiliation":[{"name":"Research Institute of Biomedical and Health Sciences University of Las Palmas de Gran Canaria 35016 Las Palmas Canary Islands Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximina","family":"Monz\u00f3n\u2010Mayor","sequence":"additional","affiliation":[{"name":"Research Institute of Biomedical and Health Sciences University of Las Palmas de Gran Canaria 35016 Las Palmas Canary Islands Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3561-09.2009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1161151"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2006.12.004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1523\/JNEUROSCI.11-03-00626.1991","article-title":"Growth of regenerating goldfish axons is inhibited by rat oligodendrocytes and CNS myelin but not but not by goldfish optic nerve tract oligodendrocytelike cells and fish CNS myelin","volume":"11","author":"Bastmeyer M","year":"1991","journal-title":"J Neurosci"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21121"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1523\/JNEUROSCI.19-02-00813.1999","article-title":"Tenascin\u2010R inhibits the growth of optic fibers in vitro but is rapidly eliminated during nerve regeneration in the salamander Pleurodeles waltl","volume":"19","author":"Becker CG","year":"1999","journal-title":"J Neurosci"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.03.003"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.04.007"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1332-9"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2006.09.004"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90212-7"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(89)90255-8"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-011-1211-9"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000219"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-59544-7.00013-5"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1541-07.2007"},{"key":"e_1_2_9_18_1","first-page":"309","article-title":"Table de d\u00e9veloppment du l\u00e9zard vivipare (Lacerta vivipara jacquin)","volume":"50","author":"Dufaure JP","year":"1961","journal-title":"Arch Anat Microsc Morphol Exp"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20299"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2006.23.371"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(86)90071-4"},{"key":"e_1_2_9_22_1","first-page":"97","article-title":"Mechanisms of CNS myelin inhibition: evidence for distinct and neuronal cell type specific receptor systems","volume":"26","author":"Giger RJ","year":"2008","journal-title":"Restor Neurol Neurosci"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2007.06.020"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23976"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023941421781"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2002.1153"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msg241"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2003.09.021"},{"key":"e_1_2_9_29_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1523\/JNEUROSCI.15-01-00099.1995","article-title":"CNS myelin and oligodendrocytes of the Xenopus spinal cord\u2014but not optic nerve\u2014are nonpermissive for axon growth","volume":"15","author":"Lang DM","year":"1995","journal-title":"J Neurosci"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199805)23:1<61::AID-GLIA6>3.0.CO;2-7"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.10099"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.20624"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2011.05.001"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.05.002"},{"key":"e_1_2_9_35_1","doi-asserted-by":"crossref","first-page":"7830","DOI":"10.1523\/JNEUROSCI.23-21-07830.2003","article-title":"Axon regeneration in goldfish and rat retinal ganglion cells: differential responsiveness to carbohydrates and cAMP","volume":"23","author":"Li Y","year":"2003","journal-title":"J Neurosci"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-162792"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986711794088326"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1188"},{"key":"e_1_2_9_39_1","first-page":"33","article-title":"An ultrastructural study of the development of astrocytes in the midbrain of the lizard","volume":"170","author":"Monz\u00f3n\u2010Mayor M","year":"1990","journal-title":"J Anat"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2010.09.002"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1432-6"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0727-08.2008"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2011.191"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(93)90243-K"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2006.05.008"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.048371"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)91345-0"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2005.08.009"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.10258"},{"key":"e_1_2_9_50_1","first-page":"54","article-title":"Radial glial cells, proliferating periventricular cells, and microglia might contribute to successful structural repair in the cerebral cortex of the lizard Gallotia galloti","volume":"57","author":"Romero\u2010Alem\u00e1n MM","year":"2004","journal-title":"Exp Neurol"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22444"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23034"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1005372107"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.20256"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.20566"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.20939"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2936"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.06.004"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903430205"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-11-07500.1995"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13064-015-0034-x"},{"key":"e_1_2_9_62_1","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.1523\/JNEUROSCI.14-03-01820.1994","article-title":"Identification and characterization of a novel (115 kDa) neurofilament\u2010associated kinase","volume":"14","author":"Xiao J","year":"1994","journal-title":"J Neurosci"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-010-1105-y"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.24112","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.24112","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.24112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T22:40:18Z","timestamp":1696632018000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.24112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,6]]},"references-count":62,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1002\/cne.24112"],"URL":"https:\/\/doi.org\/10.1002\/cne.24112","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,6]]}}}