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Twelve subunits are predicted to form anion channels gated by \u03b3\u2010aminobutyric acid (GABA), glutamate, histamine, or changes in pH, and three are putative ACh\u2010gated cation channels. Spiders have a variety of mechanosensilla and proprioceptive organs that are innervated by efferents in their peripherally located parts, and efferents also innervate muscle fibers. We investigated Cys\u2010loop gene expression in muscle tissue by qPCR and localized this expression in mechanosensilla via in situ hybridization. The cuticular mechanosensory neurons had only<jats:italic>CsGABArdl<\/jats:italic>and<jats:italic>CspHCl2<\/jats:italic>subunits, whereas the muscle tissue expressed a wider variety of subunits, especially<jats:italic>CsGABAgrd<\/jats:italic>, Cs<jats:italic>GABA<jats:sub>A<\/jats:sub>\u03b2, CsGluCl1<\/jats:italic>and<jats:italic>CspHCl<\/jats:italic>, but very low levels of the<jats:italic>CsGABArdl<\/jats:italic>or<jats:italic>CsnACh<\/jats:italic>subunits. An nACh non\u2010\u03b1 subunit was expressed in a group of unidentified cells in the hypodermis and at low level in the muscle tissue, but the physiological function of this subunit is unknown. The<jats:italic>CsnACh\u03b1<\/jats:italic>subunit was not expressed in sensory neurons and was expressed at extremely low level in the muscle tissue. None of the probes gave signals in proprioceptive joint receptors, suggesting that efferent innervation to this sense organ employs other receptor types.<jats:italic>CsAChBP<\/jats:italic>and a glia\u2010specific homeodomain<jats:italic>CsREPO<\/jats:italic>were both expressed in glial cells that surround sensory neurons and also in muscle tissue, probably around the nerve endings of the neuromuscular junction. These locations have large numbers of synapses, suggesting that AChBP may have a function in modulating synaptic transmission. J. Comp. Neurol. 525:1139\u20131154, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cne.24122","type":"journal-article","created":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T08:52:48Z","timestamp":1474447968000},"page":"1139-1154","source":"Crossref","is-referenced-by-count":8,"title":["Expression of Cys\u2010loop receptor subunits and acetylcholine binding protein in the mechanosensory neurons, glial cells, and muscle tissue of the spider<i>Cupiennius salei<\/i>"],"prefix":"10.1002","volume":"525","author":[{"given":"Hongxia","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics Dalhousie University Halifax Nova Scotia B3H 4R2 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew S.","family":"French","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics Dalhousie University Halifax Nova Scotia B3H 4R2 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