{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T20:57:12Z","timestamp":1790369832082,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,11,3]],"date-time":"2016-11-03T00:00:00Z","timestamp":1478131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01\u2010HL096750"],"award-info":[{"award-number":["R01\u2010HL096750"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32\u2010HL105355"],"award-info":[{"award-number":["T32\u2010HL105355"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Cervical spinal hemisection at C2 (SH) removes premotor drive to phrenic motoneurons located in segments C3\u2013C5 in rats. Spontaneous recovery of ipsilateral diaphragm muscle activity is associated with increased phrenic motoneuron expression of glutamatergic N\u2010methyl\u2010D\u2010aspartate (NMDA) receptors and decreased expression of \u03b1\u2010amino\u20103\u2010hydroxy\u20105\u2010methylisoxazole\u20104\u2010proprionic acid (AMPA) receptors. Glutamatergic receptor expression is regulated by tropomyosin\u2010related kinase receptor subtype B (TrkB) signaling in various neuronal systems, and increased TrkB receptor expression in phrenic motoneurons enhances recovery post\u2010SH. Accordingly, we hypothesize that recovery of ipsilateral diaphragm muscle activity post\u2010SH, whether spontaneous or enhanced by adenoassociated virus (AAV)\u2010mediated upregulation of TrkB receptor expression, is associated with increased expression of glutamatergic NMDA receptors in phrenic motoneurons. Adult male Sprague\u2010Dawley rats underwent diaphragm electromyography electrode implantation and SH surgery. Rats were injected intrapleurally with AAV expressing TrkB or GFP 3 weeks before SH. At 14 days post\u2010SH, the proportion of animals displaying recovery of ipsilateral diaphragm activity increased in AAV\u2013TrkB\u2010treated (9\/9) compared with untreated (3\/5) or AAV\u2013GFP\u2010treated (4\/10; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.027) animals. Phrenic motoneuron NMDA NR1 subunit mRNA expression was approximately fourfold greater in AAV\u2013TrkB\u2010 vs. AAV\u2013GFP\u2010treated SH animals (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.004) and in animals displaying recovery vs. those not recovering (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.005). Phrenic motoneuron AMPA glutamate receptor 2 (GluR2) subunit mRNA expression decreased after SH, and, albeit increased in animals displaying recovery vs. those not recovering, levels remained lower than control. We conclude that increased phrenic motoneuron expression of glutamatergic NMDA receptors is associated with spontaneous recovery after SH and enhanced recovery after AAV\u2013TrkB treatment. J. Comp. Neurol. 525:1192\u20131205, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cne.24125","type":"journal-article","created":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T08:52:49Z","timestamp":1474447969000},"page":"1192-1205","source":"Crossref","is-referenced-by-count":43,"title":["Motoneuron glutamatergic receptor expression following recovery from cervical spinal hemisection"],"prefix":"10.1002","volume":"525","author":[{"given":"Heather M.","family":"Gransee","sequence":"first","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic Rochester Minnesota 55905"},{"name":"Department of Anesthesiology Mayo Clinic Rochester Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A.","family":"Gonzalez Porras","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic Rochester Minnesota 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