{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T04:39:32Z","timestamp":1793248772772,"version":"4.5.0"},"reference-count":56,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T00:00:00Z","timestamp":1488499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"MEXT\/JSPS KAKENHI","award":["JP15K06748"],"award-info":[{"award-number":["JP15K06748"]}]},{"name":"MEXT\/JSPS KAKENHI","award":["JP24500418"],"award-info":[{"award-number":["JP24500418"]}]},{"name":"MEXT\/JSPS KAKENHI","award":["JP23500419"],"award-info":[{"award-number":["JP23500419"]}]},{"DOI":"10.13039\/501100009158","name":"Kawasaki Medical School","doi-asserted-by":"crossref","award":["26B-88"],"award-info":[{"award-number":["26B-88"]}],"id":[{"id":"10.13039\/501100009158","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100009158","name":"Kawasaki Medical School","doi-asserted-by":"crossref","award":["26B-43"],"award-info":[{"award-number":["26B-43"]}],"id":[{"id":"10.13039\/501100009158","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100009158","name":"Kawasaki Medical School","doi-asserted-by":"crossref","award":["26G-6"],"award-info":[{"award-number":["26G-6"]}],"id":[{"id":"10.13039\/501100009158","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mitral cells are the major projection neurons of the olfactory bulb. They receive olfactory inputs, regulate information, and project their axons to the olfactory cortex. To understand output regulation of mitral cells better, we established a method to visualize individual projection neurons and quantitatively examined their synaptic distribution. Individual mitral cells were labeled by viral injection, reconstructed three dimensionally with light microscopy, and serial sectioned for electron microscopy. Synaptic distributions were analyzed in electron microscopically reconstructed cell bodies, two regions of secondary dendrites (near the somata and \u223c200\u2009\u03bcm from the somata), and primary dendrites. The ratio of presynaptic sites (60%) and reciprocal synapses (60% presynaptic and 80% postsynaptic sites) were similar in each region. Characteristically, primary dendrite synapses were distributed mainly within the inner half of the external plexiform layer (EPL). For comparison, tufted cells were also examined, and the synaptic distribution in two secondary dendrite regions, which corresponded with mitral cells, was analyzed. The results showed that the ratio of reciprocal synapses (80% presynaptic and 90% postsynaptic sites) was greater than in mitral cells. The distribution of symmetrical synapses was also analyzed with synaptic and neuronal markers, such as parvalbumin, vesicular gamma\u2010aminobutyric acid transporter, and gephyrin. Parvalbumin\u2010expressing neurons tended to form synapses on secondary dendrites near the somata and were more uniformly distributed on primary dendrites of mitral cells. These results indicate that local mitral cell synaptic circuits are formed in accordance with their functional roles and restricted to the inner half of the EPL. J. Comp. Neurol. 525:1633\u20131648, 2017. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cne.24148","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T18:44:29Z","timestamp":1479321869000},"page":"1633-1648","source":"Crossref","is-referenced-by-count":15,"title":["Synaptic distribution of individually labeled mitral cells in the external plexiform layer of the mouse olfactory bulb"],"prefix":"10.1002","volume":"525","author":[{"given":"Takeshi","family":"Matsuno","sequence":"first","affiliation":[{"name":"Department of Anatomy Kawasaki Medical School Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emi","family":"Kiyokage","sequence":"additional","affiliation":[{"name":"Department of Anatomy Kawasaki Medical School Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Toida","sequence":"additional","affiliation":[{"name":"Department of Anatomy Kawasaki Medical School Okayama Japan"},{"name":"Research Center for Ultra\u2010High Voltage Electron Microscopy Osaka University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02185"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23714"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1523\/JNEUROSCI.04-03-00638.1984","article-title":"Effects of sensory deprivation on the developing mouse olfactory system: a light and electron microscopic, morphometric analysis","volume":"4","author":"Benson T. 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