{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:10:12Z","timestamp":1790759412336,"version":"4.1.0"},"reference-count":107,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,5,30]],"date-time":"2017-05-30T00:00:00Z","timestamp":1496102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["BFU2014\u201056517P"],"award-info":[{"award-number":["BFU2014\u201056517P"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2017,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The isthmus is recognized as the most rostral segment of the hindbrain in non\u2010mammalian vertebrates. In mammalian embryos, transient\n                    <jats:italic>Fgf8<\/jats:italic>\n                    expression defines the developing isthmic region, lying between the midbrain and the first rhombomere, but there has been uncertainty about the existence of a distinct isthmic segment in postnatal mammals. We attempted to find if the region of early embryonic\n                    <jats:italic>Fgf8<\/jats:italic>\n                    expression (which is considered to involve the entire extent of the prospective isthmus initially) might help to identify the boundaries of the isthmus in postnatal animals. By creating an\n                    <jats:italic>Fgf8<\/jats:italic>\n                    \u2010Cre\u2010LacZ lineage in mice, we were able to show that\n                    <jats:italic>Fgf8\u2010<\/jats:italic>\n                    Cre reporter expression in postnatal mice is present in the same nuclei that characterize the isthmic region in birds. The \u2018signature\u2019 isthmic structures in birds include the trochlear nucleus, the dorsal raphe nucleus, the microcellular tegmental nuclei, the pedunculotegmental nucleus, the vermis of the cerebellum, rostral parts of the parabrachial complex and locus coeruleus, and the caudal parts of the substantia nigra and VTA. We found that all of these structures were labeled with the\n                    <jats:italic>Fgf8<\/jats:italic>\n                    \u2010Cre reporter in the mouse brain, and we conclude that the isthmus is a distinct segment of the mammalian brain lying caudal to the midbrain and rostral to rhombomere 1 of the hindbrain.\n                  <\/jats:p>","DOI":"10.1002\/cne.24242","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T12:25:21Z","timestamp":1494937521000},"page":"2782-2799","source":"Crossref","is-referenced-by-count":63,"title":["Mouse\n                    <i>Fgf8<\/i>\n                    \u2010Cre\u2010LacZ lineage analysis defines the territory of the postnatal mammalian isthmus"],"prefix":"10.1002","volume":"525","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2169-5089","authenticated-orcid":false,"given":"Charles","family":"Watson","sequence":"first","affiliation":[{"name":"Neuroscience Research Australia Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomomi","family":"Shimogori","sequence":"additional","affiliation":[{"name":"RIKEN, Brain Science Institute Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Puelles","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and IMIB\u2010Arrixaca University of Murcia Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.076380"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1242\/dev.127.9.1857","article-title":"The transcription factor Lmx1b maintains Wnt1 expression within the isthmic organiser","volume":"127","author":"Adams K.","year":"2000","journal-title":"Development"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902740105"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-012-0456-8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903330411"},{"key":"e_1_2_8_7_1","first-page":"812","article-title":"Locus coeruleus neurons originate in alar rhombomere 1 and migrate into the basal plate: Studies in chick and mouse embryos","volume":"496","author":"Aroca P.","year":"2006","journal-title":"Journal of Comparative Neurology"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2004.12.031"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.011569"},{"key":"e_1_2_8_10_1","volume-title":"Neuroscience: Exploring the brain","author":"Bear M. 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