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Rat Nogo\u2010A and zebrafish Rtn4b possess characteristic motifs (M1\u20104) in the Nogo\u2010A\u2010specific region, which contains delta20, the most inhibitory region of rat Nogo\u2010A. To determine whether zebrafish M1\u20104 is inhibitory as rat M1\u20104 and Nogo\u2010A delta20, proteins were recombinantly expressed and used as substrates for zebrafish single cell RGCs, mouse hippocampal neurons and goldfish, zebrafish and chick retinal explants. When offered as homogenous substrates, neurites of hippocampal neurons and of zebrafish single cell RGCs were inhibited by zebrafish M1\u20104, rat M1\u20104, and Nogo\u2010A delta20. Neurite length increased when zebrafish single cell RGCs were treated with receptor\u2010type\u2010specific antagonists and, respectively, with morpholinos (MO) against S1PR2 and S1PR5a\u2014which represent candidate zebrafish Nogo\u2010A receptors. In a stripe assay, however, where M1\u20104 lanes alternate with polylysine\u2010(Plys)\u2010only lanes, RGC axons from goldfish, zebrafish, and chick retinal explants avoided rat M1\u20104 but freely crossed zebrafish M1\u20104 lanes\u2014suggesting that zebrafish M1\u20104 is growth permissive and less inhibitory than rat M1\u20104. Moreover, immunostainings and dot blots of optic nerve and myelin showed that expression of Rtn4b is very low in tissue and myelin at 3\u20135 days after lesion when axons regenerate. Thus, Rtn4b seems to represent no major obstacle for axon regeneration in vivo because it is less inhibitory for RGC axons from retina explants, and because of its low abundance.<\/jats:p>","DOI":"10.1002\/cne.24253","type":"journal-article","created":{"date-parts":[[2017,5,31]],"date-time":"2017-05-31T00:12:45Z","timestamp":1496189565000},"page":"2991-3009","source":"Crossref","is-referenced-by-count":10,"title":["Substrate properties of zebrafish Rtn4b\/Nogo and axon regeneration in the zebrafish optic nerve"],"prefix":"10.1002","volume":"525","author":[{"given":"Vsevolod","family":"Bodrikov","sequence":"first","affiliation":[{"name":"Department of Biology University of Konstanz Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia","family":"Welte","sequence":"additional","affiliation":[{"name":"Department of Biology University of Konstanz Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianne","family":"Wiechers","sequence":"additional","affiliation":[{"name":"Department of Biology University of Konstanz Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Weschenfelder","sequence":"additional","affiliation":[{"name":"Zoological Institute Cell and Neurobiology Biology, Karlsruhe Institute of Technology (KIT) Karlsruhe Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gurjot","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Biology University of Konstanz Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Shypitsyna","sequence":"additional","affiliation":[{"name":"Department of Biology University of Konstanz Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Pinzon\u2010Olejua","sequence":"additional","affiliation":[{"name":"Department of Biology University of Konstanz Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Bastmeyer","sequence":"additional","affiliation":[{"name":"Zoological Institute Cell and Neurobiology Biology, Karlsruhe Institute of Technology (KIT) Karlsruhe Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3817-907X","authenticated-orcid":false,"given":"Claudia A. 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