{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T01:18:31Z","timestamp":1778721511391,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,8,30]],"date-time":"2017-08-30T00:00:00Z","timestamp":1504051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Singapore Biomedical Research Council (BMRC)","award":["BMRC\/04\/1\/21\/19\/305 and 06\/1\/21\/19\/460"],"award-info":[{"award-number":["BMRC\/04\/1\/21\/19\/305 and 06\/1\/21\/19\/460"]}]},{"name":"MOE Academic Research Fund","award":["AcRF R-181-000-143-112"],"award-info":[{"award-number":["AcRF R-181-000-143-112"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Juxtanodin (JN, also known as ermin) was initially identified as an actin cytoskeleton\u2010related oligodendroglial protein in the rat central nervous system. It was subsequently also found in the rat olfactory neuroepithelium, especially at the apical junctional belt of the sustentacular cells. We further examined JN expression and functional roles in the retina using fluorescence histochemistry, confocal microscopy, immuno\u2010electron microscopy, molecular biology, and cell culture. Prominent JN expression was found in the photoreceptor\u2010supporting retinal pigment epithelium (RPE), especially in a zone corresponding to the apices of RPE cells, at the roots of the RPE microvilli, and at the base of RPE cells next to the Bruch's membrane. Partial co\u2010localization of JN immunoreactivity with F\u2010actin (labeled with phalloidin) was observed at the apices and bases of RPE cells. No JN was detected in other cell types of the retina. In cultured human RPE cell line ARPE\u201019, expression of extrinsic JN up\u2010regulated formation of actin cytoskeleton stress fibers, caused redistribution of more F\u2010actin fibers to the cell periphery, and promoted spreading\/enlargement of transfected cells. These findings suggest possible roles of JN in RPE molecular transport, phagocytosis and formation of outer blood\u2010retinal barrier, or possible involvement of JN expression perturbations in pathogenesis of such retinal disorders as proliferative vitreoretinopathy and age\u2010related macular degeneration.<\/jats:p>","DOI":"10.1002\/cne.24301","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T08:48:46Z","timestamp":1502959726000},"page":"205-215","source":"Crossref","is-referenced-by-count":9,"title":["Juxtanodin in retinal pigment epithelial cells: Expression and biological activities in regulating cell morphology and actin cytoskeleton organization"],"prefix":"10.1002","volume":"526","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1162-6212","authenticated-orcid":false,"given":"Fengyi","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Anatomy Yong Loo Lin School of Medicine, National University of Singapore Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Hyun","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Anatomy Yong Loo Lin School of Medicine, National University of Singapore Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas Weiwei","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Anatomy Yong Loo Lin School of Medicine, National University of Singapore Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter","family":"Hunziker","sequence":"additional","affiliation":[{"name":"Epithelial Cell Biology Laboratory Institute of Molecular and Cell Biology, A*STAR Singapore Singapore"},{"name":"Department of Physiology National University of Singapore Singapore Singapore"},{"name":"Singapore Eye Research Institute Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.09.015"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.7.1533"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4317-05.2006"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.07.009"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1996.0020"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01735374"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.11.007"},{"issue":"396","key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1080\/01621459.1986.10478364","article-title":"The maximum familywise error rate of Fisher's least significant difference test","volume":"81","author":"Hayter A. 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