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Friedman New York Foundation for Medical Research"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2018,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We recently reported that the number of hypothalamic tanycytes expressing pro\u2010opiomelanocortin (<jats:italic>Pomc<\/jats:italic>) is highly variable among brains of adult rats. While its cause and significance remain unknown, identifying other variably expressed genes in tanycytes may help understand this curious phenomenon. In this <jats:italic>in situ<\/jats:italic> hybridization study, we report that the <jats:italic>Prss56<\/jats:italic> gene, which encodes a trypsin\u2010like serine protease and is expressed in neural stem\/progenitor cells, shows a similarly variable mRNA expression in tanycytes of adult rats and correlates inversely with tanycyte <jats:italic>Pomc<\/jats:italic> mRNA. <jats:italic>Prss56<\/jats:italic> was expressed in \u03b11, \u03b21, subsets of \u03b12, and some median eminence \u03b3 tanycytes, but virtually absent from \u03b22 tanycytes. <jats:italic>Prss56<\/jats:italic> was also expressed in vimentin positive tanycyte\u2010like cells in the parenchyma of the ventromedial and arcuate nuclei, and in thyrotropin beta subunit\u2010expressing cells of the pars tuberalis of the pituitary. In contrast to adults, <jats:italic>Prss56<\/jats:italic> expression was uniformly high in tanycytes in adolescent rats. In mice, <jats:italic>Prss56<\/jats:italic>\u2010expressing tanycytes and parenchymal cells were also observed but fewer in number and without significant variations. The results identify <jats:italic>Prss56<\/jats:italic> as a second gene that is expressed variably in tanycytes of adult rats. We propose that the variable, inversely correlating expression of <jats:italic>Prss56<\/jats:italic> and <jats:italic>Pomc<\/jats:italic> reflect periodically oscillating gene expression in tanycytes rather than stable expression levels that vary between individual rats. A possible functional link between Prss56 and POMC, and <jats:italic>Prss56<\/jats:italic> as a potential marker for migrating tanycytes are discussed.<\/jats:p>","DOI":"10.1002\/cne.24504","type":"journal-article","created":{"date-parts":[[2018,9,22]],"date-time":"2018-09-22T13:16:54Z","timestamp":1537622214000},"page":"2444-2461","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<i>Prss56<\/i> expression in the rodent hypothalamus: Inverse correlation with pro\u2010opiomelanocortin suggests oscillatory gene expression in adult rat tanycytes"],"prefix":"10.1002","volume":"526","author":[{"given":"G\u00e1bor","family":"Wittmann","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Endocrinology Diabetes and Metabolism, Tupper Research Institute, Tufts Medical Center  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald M.","family":"Lechan","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Endocrinology Diabetes and Metabolism, Tupper Research Institute, Tufts Medical Center  Boston Massachusetts"},{"name":"Department of Neuroscience Tufts University School of Medicine  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(20000403)419:2<244::AID-CNE8>3.0.CO;2-R"},{"issue":"3","key":"e_1_2_6_3_1","first-page":"407","article-title":"Morphological aspects of the hypothalamic\u2010hypophyseal system. 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