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[18<\/jats:sup>F]FEOBV binding was highest in striatum, intermediate in the amygdala, hippocampal formation, thalamus, rostral brainstem, some cerebellar regions, and lower in other regions. Neocortical [18<\/jats:sup>F]FEOBV binding was inhomogeneous with relatively high binding in insula, BA24, BA25, BA27, BA28, BA34, BA35, pericentral cortex, and lowest in BA17\u201319. Thalamic [18<\/jats:sup>F]FEOBV binding was inhomogeneous with greatest binding in the lateral geniculate nuclei and relatively high binding in medial and posterior thalamus. Cerebellar cortical [18<\/jats:sup>F]FEOBV binding was high in vermis and flocculus, and lower in the lateral cortices. Brainstem [18<\/jats:sup>F]FEOBV binding was most prominent at the mesopontine junction, likely associated with the pedunculopontine\u2013laterodorsal tegmental complex. Significant [18<\/jats:sup>F]FEOBV binding was present throughout the brainstem. Some regions, including the striatum, primary sensorimotor cortex, and anterior cingulate cortex exhibited age\u2010related decreases in [18<\/jats:sup>F]FEOBV binding. These results are consistent with prior studies of cholinergic projections in other species and prior postmortem human studies. There is a distinctive pattern of human neocortical VChAT expression. The patterns of thalamic and cerebellar cortical cholinergic terminal distribution are likely unique to humans. Normal aging is associated with regionally specific reductions in [18<\/jats:sup>F]FEOBV binding in some cortical regions and the striatum.<\/jats:p>","DOI":"10.1002\/cne.24541","type":"journal-article","created":{"date-parts":[[2018,9,26]],"date-time":"2018-09-26T10:58:23Z","timestamp":1537959503000},"page":"2884-2897","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Regional vesicular acetylcholine transporter distribution in human brain: A [18<\/sup>F]fluoroethoxybenzovesamicol positron emission tomography study"],"prefix":"10.1002","volume":"526","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0629-608X","authenticated-orcid":false,"given":"Roger L.","family":"Albin","sequence":"first","affiliation":[{"name":"Neurology Service & GRECC, VAAAHS Ann Arbor Michigan"},{"name":"Department of Neurology University of Michigan Ann Arbor Michigan"},{"name":"University of Michigan Morris K. 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