{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T15:44:52Z","timestamp":1758815092501,"version":"3.40.5"},"reference-count":46,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T00:00:00Z","timestamp":1540857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21611009","25282250","25610126"],"award-info":[{"award-number":["21611009","25282250","25610126"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Multiple sclerosis is a neurological disorder in which the myelin sheaths of axons are damaged by the immune response. We report here a three\u2010dimensional structural analysis of brain and spinal cord tissues of a mouse model of multiple sclerosis, known as experimental autoimmune encephalomyelitis (EAE). EAE\u2010induced mice were raised with or without administration of fingolimod, which is used in the treatment of multiple sclerosis. Brains and spinal cords dissected from the EAE mice were lyophilized so as to reconstitute the intrinsic contrast of tissue elements, such as axons, in X\u2010ray images. Three\u2010dimensional structures of the brain hemispheres and spinal cords of the EAE mice were visualized with synchrotron radiation microtomography. Microtomographic cross sections reconstructed from the X\u2010ray images revealed dilation of capillary vessels and vacuolation in the spinal cord of the EAE mice. Vacuolation was also observed in the cerebellum, suggesting that the neuroinflammatory response progressed in the brain. The vessel networks and vacuolation lesions in the spinal cords were modelled by automatically tracing the three\u2010dimensional image in order to analyze the tissue structures quantitatively. The results of the analysis indicated that the distribution of vacuolations was not uniform but three\u2010dimensionally localized. The mean vessel diameter showed a linear correlation with the clinical score, indicating that vasodilation is relevant to paralysis severity in the disease model. We suggest that vasodilation and vacuolation are related with neurological symptoms of multiple sclerosis.<\/jats:p>","DOI":"10.1002\/cne.24544","type":"journal-article","created":{"date-parts":[[2018,10,6]],"date-time":"2018-10-06T16:29:01Z","timestamp":1538843341000},"page":"2091-2100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Synchrotron radiation microtomography of brain hemisphere and spinal cord of a mouse model of multiple sclerosis revealed a correlation between capillary dilation and clinical score"],"prefix":"10.1002","volume":"527","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7427-7764","authenticated-orcid":false,"given":"Rino","family":"Saiga","sequence":"first","affiliation":[{"name":"Department of Applied Biochemistry Tokai University  Hiratsuka Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Hoshino","sequence":"additional","affiliation":[{"name":"Japan Synchrotron Radiation Research Institute (JASRI\/SPring\u20108)  Sayo Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihisa","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Japan Synchrotron Radiation Research Institute (JASRI\/SPring\u20108)  Sayo Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kentaro","family":"Uesugi","sequence":"additional","affiliation":[{"name":"Japan Synchrotron Radiation Research Institute (JASRI\/SPring\u20108)  Sayo Hyogo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuko","family":"Naitou","sequence":"additional","affiliation":[{"name":"Support Center for Medical Research and Education Tokai University  Isehara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akemi","family":"Kamijo","sequence":"additional","affiliation":[{"name":"Support Center for Medical Research and Education Tokai University  Isehara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noboru","family":"Kawabe","sequence":"additional","affiliation":[{"name":"Support Center for Medical Research and Education Tokai University  Isehara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Ohtsuka","sequence":"additional","affiliation":[{"name":"Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine Tokai University School of Medicine  Isehara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4567-6384","authenticated-orcid":false,"given":"Shunya","family":"Takizawa","sequence":"additional","affiliation":[{"name":"Department of Neurology Tokai University School of Medicine  Isehara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5484-4861","authenticated-orcid":false,"given":"Ryuta","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Department of Applied Biochemistry Tokai University  Hiratsuka Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12041"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2007.06.023"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119356"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/123.6.1174"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(02)00069-2"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C200176200"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21307"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ahj.2014.06.028"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-06251-7"},{"issue":"10","key":"e_1_2_8_11_1","doi-asserted-by":"crossref","first-page":"5037","DOI":"10.4049\/jimmunol.160.10.5037","article-title":"FTY720, a novel immunosuppressant, induces sequestration of circulating mature lymphocytes by acceleration of lymphocyte homing in rats. 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Saiga R. Takeuchi A. Uesugi K. Terada Y. Suzuki Y. De Andrade V. \u2026Arai M.(2018). Three\u2010dimensional alteration of neurons in schizophrenia. 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