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Olfactory ensheathing cells (OECs) are specialized glia associated with olfactory sensory neuron axons. Like other glia, they form selective networks, however, the connexins that support OEC connectivity in vivo have not been identified. We used an in vivo mouse model to selectively delete candidate connexin genes with temporal control from OECs and address the physiological consequences. Using this model, we effectively abolished the expression of connexin 43 (Cx43) in OECs in both juvenile and adult mice. Cx43\u2010deleted OECs exhibited features consistent with the loss of gap junctions including reduced membrane conductance, largely reduced sensitivity to the gap junction blocker meclofenamic acid and loss of dye coupling. This indicates that Cx43, a typically astrocytic connexin, is the main connexin forming functional channels in OECs. Despite these changes in functional properties, the deletion of Cx43 deletion did not alter the density of OECs. The strategy used here may prove useful to delete other candidate genes to better understand the functional roles of OECs in vivo.<\/jats:p>","DOI":"10.1002\/cne.24628","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T04:46:44Z","timestamp":1547095604000},"page":"1278-1289","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Selective Cre\u2010mediated gene deletion identifies connexin 43 as the main connexin channel supporting olfactory ensheathing cell networks"],"prefix":"10.1002","volume":"527","author":[{"given":"Ana Paula","family":"Piantanida","sequence":"first","affiliation":[{"name":"CONICET, Instituto de Fisiolog\u00eda y Biof\u00edsica Bernardo Houssay (IFIBIO), Neurociencia de Sistemas  Buenos Aires Argentina"},{"name":"Universidad de Buenos Aires, Instituto de Fisiolog\u00eda y Biof\u00edsica Bernardo Houssay (IFIBIO), Neurociencia de Sistemas  Buenos Aires 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