{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T12:56:28Z","timestamp":1791636988602,"version":"4.3.4"},"reference-count":27,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2019,3,21]],"date-time":"2019-03-21T00:00:00Z","timestamp":1553126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We aimed to investigate the effect of chronic cerebral hypoperfusion on cerebral hemodynamics and perivascular nerve density in a rat model. Bilateral common carotid artery (CCA) ligation (<jats:italic>n<\/jats:italic>\u2009=\u200924) or sham\u2010operation (<jats:italic>n<\/jats:italic>\u2009=\u200924) was performed with a 1\u2010week interval. A subgroup (ligated <jats:italic>n<\/jats:italic>\u2009=\u20096; sham\u2010operated <jats:italic>n<\/jats:italic>\u2009=\u20093) underwent magnetic resonance imaging (MRI) before the procedures and 2 and 4\u2009weeks after the second procedure. After termination, carotids were harvested for assessment of complete ligation and nerve density in cerebral arteries that were stained for the general neural marker PGP 9.5 and sympathetic marker TH by computerized image analysis. Five rats were excluded because of incomplete ligation. MRI\u2010based tortuosity of the posterior communicating artery (Pcom), first part of the posterior cerebral artery (P1) and basilar artery was observed in the ligated group, as well as an increased volume (<jats:italic>p<\/jats:italic>\u2009=\u20090.05) and relative signal intensity in the basilar artery (<jats:italic>p<\/jats:italic>\u2009=\u20090.04; sham\u2010group unchanged). Immunohistochemical analysis revealed that compared to sham\u2010operated rats, ligated rats had increased diameters of all intracircular segments and the extracircular part of the internal carotid artery (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Ligated rats showed a higher general nerve density compared to controls in P1 (10%, IQR:8.7\u201310.5 vs. 6.6%, IQR:5.5\u20137.4, <jats:italic>p<\/jats:italic>\u2009=\u20090.003) and Pcom segments (6.4%, IQR:5.8\u20136.5 vs. 3.2%, IQR:2.4\u20134.3, <jats:italic>p<\/jats:italic>\u2009=\u20090.003) and higher sympathetic nerve density in Pcom segments (3.7%, IQR:2.8\u20134.8 vs. 1.7%, IQR:1.3\u20132.2, <jats:italic>p<\/jats:italic>\u2009=\u20090.02). Bilateral CCA occlusion resulted in redistribution of blood flow to posteriorly located cerebral arteries with remarkable changes in morphology and perivascular nerve density, suggesting a functional role for perivascular nerves in cerebral autoregulation.<\/jats:p>","DOI":"10.1002\/cne.24672","type":"journal-article","created":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T17:47:00Z","timestamp":1551894420000},"page":"2263-2272","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effect of bilateral carotid occlusion on cerebral hemodynamics and perivascular innervation: An experimental rat model"],"prefix":"10.1002","volume":"527","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3751-0376","authenticated-orcid":false,"given":"M. L.","family":"Rots","sequence":"first","affiliation":[{"name":"Department of Vascular Surgery University Medical Center Utrecht, Utrecht University  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. J.","family":"de Borst","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery University Medical Center Utrecht, Utrecht University  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"van der Toorn","sequence":"additional","affiliation":[{"name":"Center for Image Sciences University Medical Center Utrecht, Utrecht University  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. L.","family":"Moll","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery University Medical Center Utrecht, Utrecht University  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. W. A.","family":"Pennekamp","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery University Medical Center Utrecht, Utrecht University  Utrecht The Netherlands"},{"name":"Department of Anatomy University Medical Center Utrecht, Utrecht University  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. M.","family":"Dijkhuizen","sequence":"additional","affiliation":[{"name":"Center for Image Sciences University Medical Center Utrecht, Utrecht University  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. L. A. W.","family":"Bleys","sequence":"additional","affiliation":[{"name":"Department of Anatomy University Medical Center Utrecht, Utrecht University  Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199609000-00030"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199609000-00030"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1075"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2010007500124"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600259"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","DOI":"10.4199\/C00005ED1V01Y200912ISP002","volume-title":"The cerebral circulation","author":"Cipolla M. 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