{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:13:48Z","timestamp":1790381628644,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T00:00:00Z","timestamp":1559865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chemosensitivity is a key mechanism for the regulation of breathing in vertebrates. The retrotrapezoid nucleus is a crucial hub for respiratory chemoreception within the brainstem. It integrates chemosensory information that are both peripheral from the carotid bodies (via the nucleus of the solitary tract) and central through the direct sensing of extracellular protons. To date, the location of a genetically defined RTN has only been ascertained in rodents. We first demonstrated that Phox2b, a key determinant for the development of the visceral nervous system and branchiomotor nuclei in the brainstem including the RTN, had a similar distribution in the brainstem of adult macaques compared to adult rats. Second, based on previous description of a specific molecular signature for the RTN in rats, and on an innovative technique for duplex in situ hybridization, we identified parafacial neurons which coexpressed <jats:italic>Phox2b<\/jats:italic> and <jats:italic>ppGal<\/jats:italic> mRNAs. They were located ventrally to the nucleus of the facial nerve and extended from the caudal part of the nucleus of the superior olive to the rostral tip of the inferior olive. Using the previously described blockface technique, deformations were corrected to allow the proper alignment and stacking of digitized sections, hence providing for the first time a 3D reconstruction of the macaque brainstem, Phox2b distribution and the primate retrotrapezoid nucleus. This description should help bridging the gap between rodents and humans for the description of key respiratory structures in the brainstem.<\/jats:p>","DOI":"10.1002\/cne.24713","type":"journal-article","created":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T17:42:39Z","timestamp":1557423759000},"page":"2875-2884","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Tridimensional mapping of Phox2b expressing neurons in the brainstem of adult <i>Macaca fascicularis<\/i> and identification of the retrotrapezoid nucleus"],"prefix":"10.1002","volume":"527","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1388-0724","authenticated-orcid":false,"given":"Jonathan","family":"Levy","sequence":"first","affiliation":[{"name":"INSERM UMR1179\u2014Handicap Neuromusculaire Universit\u00e9 de Versailles Saint\u2010Quentin\u2010en\u2010Yvelines  Montigny\u2010le\u2010Bretonneux France"},{"name":"Service de M\u00e9decine Physique et de R\u00e9adaptation\u2014APHP H\u00f4pital Raymond Poincar\u00e9  Garches France"},{"name":"Fondation Garches\u2014APHP H\u00f4pital Raymond Poincar\u00e9  Garches France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Facchinetti","sequence":"additional","affiliation":[{"name":"INSERM UMR1179\u2014Handicap Neuromusculaire Universit\u00e9 de Versailles Saint\u2010Quentin\u2010en\u2010Yvelines  Montigny\u2010le\u2010Bretonneux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Jan","sequence":"additional","affiliation":[{"name":"Molecular Imaging Research Center (MIRCen)\u2014Commissariat \u00e0 l'\u00c9nergie Atomique (CEA)  Fontenay\u2010aux\u2010Roses France"},{"name":"CNRS\u2010CEA UMR9199\u2014Neurodegenerative Diseases Laboratory Universit\u00e9 Paris\u2010Saclay  Fontenay\u2010aux\u2010Roses France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lyna","family":"Achour","sequence":"additional","affiliation":[{"name":"INSERM UMR1179\u2014Handicap Neuromusculaire Universit\u00e9 de Versailles Saint\u2010Quentin\u2010en\u2010Yvelines  Montigny\u2010le\u2010Bretonneux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9ment","family":"Bouvier","sequence":"additional","affiliation":[{"name":"Molecular Imaging Research Center (MIRCen)\u2014Commissariat \u00e0 l'\u00c9nergie Atomique (CEA)  Fontenay\u2010aux\u2010Roses France"},{"name":"NEOXIA  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Fran\u00e7ois","family":"Brunet","sequence":"additional","affiliation":[{"name":"Institut de Biologie de l'Ecole Normale Sup\u00e9rieure (IBENS), Ecole normale sup\u00e9rieure, CNRS, INSERM, PSL Universit\u00e9  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Delzescaux","sequence":"additional","affiliation":[{"name":"Molecular Imaging Research Center (MIRCen)\u2014Commissariat \u00e0 l'\u00c9nergie Atomique (CEA)  Fontenay\u2010aux\u2010Roses France"},{"name":"CNRS\u2010CEA UMR9199\u2014Neurodegenerative Diseases Laboratory Universit\u00e9 Paris\u2010Saclay  Fontenay\u2010aux\u2010Roses France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Giuliano","sequence":"additional","affiliation":[{"name":"INSERM UMR1179\u2014Handicap Neuromusculaire Universit\u00e9 de Versailles Saint\u2010Quentin\u2010en\u2010Yvelines  Montigny\u2010le\u2010Bretonneux France"},{"name":"Service de M\u00e9decine Physique et de R\u00e9adaptation\u2014APHP H\u00f4pital Raymond Poincar\u00e9  Garches France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1090940210"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1130"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2017.09.007"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3171\/2008.3.17469"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22769"},{"key":"e_1_2_8_7_1","unstructured":"Bouvier C. 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