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Injury, resulting in VF paralysis, may contribute to subsequent swallowing, voice, and respiratory dysfunction. Unfortunately, treatment for RLN injury does little to restore physiologic function of the VFs. Thus, we sought to create a mouse model with translational functional outcomes to further investigate RLN regeneration and potential therapeutic interventions. To do so, we performed ventral neck surgery in 21 C57BL\/6J male mice, divided into two groups: Unilateral RLN Transection (<jats:italic>n<\/jats:italic> = 11) and Sham Injury (<jats:italic>n<\/jats:italic> = 10). Mice underwent behavioral assays to determine upper airway function at multiple time points prior to and following surgery. Transoral endoscopy, videofluoroscopy, ultrasonic vocalizations, and whole\u2010body plethysmography were used to assess VF motion, swallow function, vocal function, and respiratory function, respectively. Affected outcome metrics, such as VF motion correlation, intervocalization interval, and peak inspiratory flow were identified to increase the translational potential of this model. Additionally, immunohistochemistry was used to investigate neuronal cell death in the nucleus ambiguus. Results revealed that RLN transection created ipsilateral VF paralysis that did not recover by 13\u2009weeks postsurgery. Furthermore, there was evidence of significant vocal and respiratory dysfunction in the RLN transection group, but not the sham injury group. No significant differences in swallow function or neuronal cell death were found between the two groups. In conclusion, our mouse model of RLN injury provides several novel functional outcome measures to increase the translational potential of findings in preclinical animal studies. We will use this model and behavioral assays to assess various treatment options in future studies.<\/jats:p>","DOI":"10.1002\/cne.24774","type":"journal-article","created":{"date-parts":[[2019,9,12]],"date-time":"2019-09-12T06:57:45Z","timestamp":1568271465000},"page":"574-596","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Recurrent laryngeal nerve transection in mice results in translational upper airway dysfunction"],"prefix":"10.1002","volume":"528","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8835-6906","authenticated-orcid":false,"given":"Megan M.","family":"Haney","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology University of Missouri  Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Hamad","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering and Computer Science University of Missouri  Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henok G.","family":"Woldu","sequence":"additional","affiliation":[{"name":"Department of Health Management &amp; Informatics University of Missouri  Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Ciucci","sequence":"additional","affiliation":[{"name":"Department of Communication Sciences and Disorders University of Wisconsin\u2013Madison  Madison Wisconsin"},{"name":"Department of Surgery, Division of Otolaryngology University of Wisconsin\u2010Madison  Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Nichols","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Missouri  Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filiz","family":"Bunyak","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering and Computer Science University of Missouri  Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa E.","family":"Lever","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Missouri  Columbia Missouri"},{"name":"Department of Otolaryngology\u2013Head and Neck Surgery University of Missouri  Columbia Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,18]]},"reference":[{"issue":"1","key":"e_1_2_8_2_1","first-page":"89","article-title":"A surgical mouse model of iatrogenic laryngeal nerve injury","volume":"155","author":"Allen J.","year":"2016","journal-title":"Otolaryngology \u2010 Head and Neck Surgery"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0033-1354253"},{"issue":"6","key":"e_1_2_8_4_1","first-page":"245","article-title":"Swallowing evaluation in patients with unilateral vocal fold immobility","volume":"3","author":"Aneas G. 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