{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T11:50:36Z","timestamp":1781610636587,"version":"3.54.5"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T00:00:00Z","timestamp":1572912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006377","name":"Purdue University","doi-asserted-by":"publisher","award":["Salary Savings"],"award-info":[{"award-number":["Salary Savings"]}],"id":[{"id":"10.13039\/100006377","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vagal afferents innervating the small intestinal mucosa regulate feeding, gastrointestinal (GI) digestive, and immune functions. Their anatomical\u2010functional characterization has been impeded by the inability to selectively label and manipulate them. Na<jats:sub>v<\/jats:sub>1.8\u2010Cre\u2010tdTomato mice label 80% of nodose and dorsal root ganglia neurons. Here, the origin of these neuron's terminals and their distribution in the small intestinal mucosa were examined by quantitatively comparing tdTomato\u2010labeled innervation in nonoperated (control), subdiaphragmatic vagotomy (VAGX), and sham\u2010operated mice. Control mice exhibited a large proximal\u2010to\u2010distal decrease and a moderate mesentery\u2010to\u2010antimesentery decrease in villus innervation. VAGX reduced this innervation to a greater degree proximally (91\u201393%) than distally (65\u201372%), resulting in flat proximal\u2010distal distributions. Therefore, estimates of vagal villus afferent distributions (control minus VAGX) paralleled control distributions, but were slightly reduced in magnitude. Compared with villus afferents, crypt innervation exhibited a muted proximal\u2010to\u2010distal decrease in control mice and a smaller loss after VAGX (45\u201348%). Sham\u2010operated mice exhibited similar distributions of villus and crypt afferents as control mice, suggesting surgery did not contribute to the effects of VAGX. Most crypt and villus afferent terminals along the entire proximal\u2010distal small intestinal axis had similar morphology to those previously reported in the proximal duodenum, but the density of terminal branches varied. Our findings suggest the majority of small intestinal mucosal innervation labeled in Na<jats:sub>v<\/jats:sub>1.8\u2010Cre\u2010tdTomato mice is vagal in origin. Therefore, these mice will be valuable for studying vagal mucosal afferent morphology, interactions with other GI elements, plasticity, and function.<\/jats:p>","DOI":"10.1002\/cne.24791","type":"journal-article","created":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T22:31:46Z","timestamp":1571265106000},"page":"816-839","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Abdominal vagotomy reveals majority of small intestinal mucosal afferents labeled in na<sub>v<\/sub>1.8cre\u2010rosa26tdTomato mice are vagal in origin"],"prefix":"10.1002","volume":"528","author":[{"given":"Hannah K.","family":"Serlin","sequence":"first","affiliation":[{"name":"Behavioral Neurogenetics Laboratory, Department of Psychological Sciences Purdue University  West Lafayette Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8439-7559","authenticated-orcid":false,"given":"Edward A.","family":"Fox","sequence":"additional","affiliation":[{"name":"Behavioral Neurogenetics Laboratory, Department of Psychological Sciences Purdue University  West Lafayette Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00187819"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000147837"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050037"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1042-14.2014"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01452"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2012.02326.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2018.01.012"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/69.1.6"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00420.2004"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00015.2003"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2016.01.041"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1984.sp015390"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/62.9.968"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00095.2017"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-012-9637-8"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.279.3.R997"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M400030-JLR200"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0330302"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2018.03.016"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00239140"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-002-0678-9"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2006.03.005"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-21-08602.2001"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-002-0261-x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20001218)428:3<558::AID-CNE11>3.0.CO;2-M"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290100184"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050094"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4476"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(86)90023-8"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23376"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22667"},{"issue":"4","key":"e_1_2_10_33_1","first-page":"G625","article-title":"Intestinal lipid inhibits gastric emptying via CCK and a vagal capsaicin\u2010sensitive afferent pathway in rats","volume":"267","author":"Holzer H. 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