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Despite copious benefits granted by these experimental advances, the sensory physiognomy of these organisms has remained poorly understood. Therefore, the present study evaluates sensory and neuronal response properties of animal models developed for the study of optically induced acetylcholine release regulation. Auditory brainstem responses, fluorescence imaging, and patch clamp recording techniques were used to assess the impact of viral infection, sex, age, and anesthetic agents across the ascending auditory pathway of ChAT\u2010Cre and ChAT\u2010ChR2(Ai32) mice. Data analyses revealed that neither genetic configuration nor adeno\u2010associated viral infection alters the early stages of auditory processing or the cellular response properties of cholinergic neurons. However, anesthetic agent and dosage amount profoundly modulate the response properties of brainstem neurons. Last, analyses of age\u2010related hearing loss in virally infected ChAT\u2010Cre mice did not differ from those reported in wild type animals. This investigation demonstrates that ChAT\u2010Cre and ChAT\u2010ChR2(Ai32) mice are viable models for the study of cholinergic modulation in auditory processing, and it emphasizes the need for prudence in the selection of anesthetic procedures.<\/jats:p>","DOI":"10.1002\/cne.24795","type":"journal-article","created":{"date-parts":[[2019,10,18]],"date-time":"2019-10-18T09:14:59Z","timestamp":1571390099000},"page":"787-804","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multiparametric assessment of the impact of opsin expression and anesthesia on striatal cholinergic neurons and auditory brainstem activity"],"prefix":"10.1002","volume":"528","author":[{"given":"Andres","family":"Carrasco","sequence":"first","affiliation":[{"name":"Neurobiology Research Unit Okinawa Institute of Science and Technology Graduate University  Onna Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Tamura","sequence":"additional","affiliation":[{"name":"Neurobiology Research Unit Okinawa Institute of Science and Technology Graduate University  Onna Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Pommer","sequence":"additional","affiliation":[{"name":"Neurobiology Research Unit Okinawa Institute of Science and Technology Graduate University  Onna Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie A.","family":"Chouinard","sequence":"additional","affiliation":[{"name":"Neurobiology Research Unit Okinawa Institute of Science and Technology Graduate University  Onna Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoto","family":"Kurima","sequence":"additional","affiliation":[{"name":"Neurobiology Research Unit Okinawa Institute of Science and Technology Graduate University  Onna Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Barzaghi","sequence":"additional","affiliation":[{"name":"Imaging Section Okinawa Institute of Science and Technology Graduate University  Onna Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8795-1209","authenticated-orcid":false,"given":"Jeffery R.","family":"Wickens","sequence":"additional","affiliation":[{"name":"Neurobiology Research Unit Okinawa Institute of Science and Technology Graduate University  Onna Okinawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_9_2_1","DOI":"10.1016\/j.nbd.2017.10.010"},{"volume-title":"The auditory brain stem responses","year":"2000","author":"Arnold S. 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