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While its cytoarchitecture and overall efferent projections are known, we lack comprehensive information on the projection patterns of specific neuronal subtypes in the PB. In this study, we compared the projection patterns of glutamatergic neurons here with a subpopulation expressing the transcription factor\n                    <jats:italic>Foxp2<\/jats:italic>\n                    and a further subpopulation expressing the neuropeptide\n                    <jats:italic>Pdyn<\/jats:italic>\n                    . To do this, we injected an AAV into the PB region to deliver a Cre\u2010dependent anterograde tracer (synaptophysin\u2010mCherry) in three different strains of Cre\u2010driver mice. We then analyzed 147 neuroanatomical regions for labeled boutons in every brain (\n                    <jats:italic>n<\/jats:italic>\n                    =\u200911). Overall, glutamatergic neurons in the PB region project to a wide variety of sites in the cerebral cortex, basal forebrain, bed nucleus of the stria terminalis, amygdala, diencephalon, and brainstem.\n                    <jats:italic>Foxp2<\/jats:italic>\n                    and\n                    <jats:italic>Pdyn<\/jats:italic>\n                    subpopulations project heavily to the hypothalamus, but not to the cortex, basal forebrain, or amygdala. Among the few differences between\n                    <jats:italic>Foxp2<\/jats:italic>\n                    and\n                    <jats:italic>Pdyn<\/jats:italic>\n                    cases was a notable lack of\n                    <jats:italic>Pdyn<\/jats:italic>\n                    projections to the ventromedial hypothalamic nucleus. Our results indicate that genetic identity determines connectivity (and therefore, function), providing a framework for mapping all PB output projections based on the genetic identity of its neurons. Using genetic markers to systematically classify PB neurons and their efferent projections will enhance the translation of research findings from experimental animals to humans.\n                  <\/jats:p>","DOI":"10.1002\/cne.24975","type":"journal-article","created":{"date-parts":[[2020,7,4]],"date-time":"2020-07-04T12:25:01Z","timestamp":1593865501000},"page":"657-693","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Efferent projections of\n                    <scp>Vglut2<\/scp>\n                    ,\n                    <scp>Foxp2<\/scp>\n                    , and\n                    <scp>Pdyn<\/scp>\n                    parabrachial neurons in mice"],"prefix":"10.1002","volume":"529","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2063-8995","authenticated-orcid":false,"given":"Dake","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Neurology University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fillan S.","family":"Grady","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lila","family":"Peltekian","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9956-4006","authenticated-orcid":false,"given":"Joel C.","family":"Geerling","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903410302"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24853"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903290205"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903530403"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19970707)383:3<245::AID-CNE1>3.0.CO;2-3"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19990322)405:4<421::AID-CNE1>3.0.CO;2-2"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.73.2.568"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.1998.19330417.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00830-9"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25448"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02284844"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25511"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)91087-W"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3729-14.2015"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12596"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903260207"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-11-06500.1994"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.09.032"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.12822"},{"issue":"42","key":"e_1_2_10_21_1","doi-asserted-by":"crossref","first-page":"8225","DOI":"10.1523\/JNEUROSCI.1162-19.2019","article-title":"Parabrachial complex: A hub for pain and aversion","volume":"39","author":"Chiang M. 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