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Abnormal densities or patterns of serotonergic fibers have been implicated in neuropsychiatric disorders. Although many classical studies have examined the distribution pattern of serotonergic fibers, most of them were either limited to specific brain areas or had limitations in demonstrating the fine axonal morphology. In this study, we utilize male mice expressing green fluorescence protein under the serotonin transporter (SERT) promoter to map the topography of serotonergic fibers across the rostro\u2010caudal extent of each brain area. We demonstrate previously unreported regional density and fine\u2010grained anatomy of serotonergic fibers. Our findings include: (a) SERT fibers distribute abundantly in the thalamic nuclei close to the midline and dorsolateral areas, in most of the hypothalamic nuclei with few exceptions such as the median eminence and arcuate nuclei, and within the basal amygdaloid complex and lateral septal nuclei, (b) the source fibers of innervation of the hippocampus traverse through the septal nuclei before reaching its destination, (c) unique, filamentous type of straight terminal fibers within the nucleus accumbens, (d) laminar pattern of innervation in the hippocampus, olfactory bulb and cortex with heterogenicity in innervation density among the layers, (e) cortical labeling density gradually decreases rostro\u2010caudally, (f) fibers traverse and distribute mostly within the gray matter, leaving the white fiber bundles uninnervated, and (g) most of the highly labeled nuclei and cortical areas have predominant anatomical connection to limbic structures. In conclusion, we provide novel, regionally specific insights on the distribution map of serotonergic fibers using transgenic mouse.<\/jats:p>","DOI":"10.1002\/cne.25027","type":"journal-article","created":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T05:41:06Z","timestamp":1599370866000},"page":"1391-1429","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Comprehensive topographical map of the serotonergic fibers in the male mouse brain"],"prefix":"10.1002","volume":"529","author":[{"given":"Janak R.","family":"Awasthi","sequence":"first","affiliation":[{"name":"RIKEN Brain Science Institute  Wako Japan"},{"name":"Graduate School of Science and Engineering Saitama University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kota","family":"Tamada","sequence":"additional","affiliation":[{"name":"RIKEN Brain Science Institute  Wako Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric T. 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