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P2X3 immunoreactivity was observed in subserosal nerve endings proximal and lateral to the gastric sling muscles in the distal antrum of the lesser curvature. Parent axons ramified into several lamellar processes to form net\u2010like complex structures that extended two\u2010dimensionally in every direction on the surface of the longitudinal smooth muscle layer. The axon terminals in the periphery of P2X3\u2010immunoreactive net\u2010like structures were flat and looped or leaf\u2010like in shape. Some net\u2010like lamellar structures and their axon terminals with P2X3 immunoreactivity were also immunoreactive for P2X2. P2X3\u2010immunoreactive nerve fibers forming net\u2010like terminal structures were closely surrounded by S100B\u2010immunoreactive terminal Schwann cells, whereas axon terminals twined around these cells and extended club\u2010, knob\u2010, or thread\u2010like protrusions in the surrounding tissues. Furthermore, a retrograde tracing method using fast blue dye indicated that most of these nerve endings originated from the nodose ganglia of the vagus nerve. These results suggest that P2X3\u2010immunoreactive subserosal nerve endings have morphological characteristics of mechanoreceptors and contribute to sensation of a mechanical deformation of the distal antral wall associated with antral peristalsis.<\/jats:p>","DOI":"10.1002\/cne.25069","type":"journal-article","created":{"date-parts":[[2020,11,15]],"date-time":"2020-11-15T05:43:51Z","timestamp":1605419031000},"page":"2014-2028","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Distribution and morphology of\n                    <scp>P2X3<\/scp>\n                    \u2010immunoreactive subserosal afferent nerve endings in the rat gastric antrum"],"prefix":"10.1002","volume":"529","author":[{"given":"Masato","family":"Hirakawa","sequence":"first","affiliation":[{"name":"Department of Anatomy (Cell Biology) Iwate Medical University  Yahaba\u2010cho 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