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While the origin of the cell bodies of uterine spinal afferents is clear, the identity of their sensory endings has remained unknown. Hence, our major aim was to identify the location, morphology, and calcitonin gene\u2010related peptide (CGRP)\u2010immunoreactivity of uterine spinal afferent endings supplied by thoracolumbar DRG. We also sought to determine the degree of uterine afferent innervation provided by the vagus nerve. Using an anterograde tracing technique, nulliparous female C57BL\/6 mice were injected unilaterally with biotinylated dextran into thoracolumbar DRG (T13\u2010L3). After 7\u20139\u2009days, uterine horns were stained to visualize traced nerve axons and endings immunoreactive to CGRP. Whole uteri from a separate cohort of animals were injected with retrograde neuronal tracer (DiI) and dye uptake in nodose ganglia was examined. Anterogradely labeled axons innervated each uterine horn, these projected rostrally or caudally from their site of entry, branching to form varicose endings in the myometrium and\/or vascular plexus. Most spinal afferent endings were CGRP\u2010immunoreactive and morphologically classified as \u201csimple\u2010type.\u201d Rarely, uterine nerve cell bodies were labeled in nodose ganglia. Here, we provide the first detailed description of spinal afferent nerve endings in the uterus of a vertebrate. Distinct morphological types of spinal afferent nerve endings were identified throughout multiple anatomical layers of the uterine wall. Compared to other visceral organs, uterine spinal afferent endings displayed noticeably less morphological diversity. Few neurons in nodose ganglia innervate the uterus.<\/jats:p>","DOI":"10.1002\/cne.25070","type":"journal-article","created":{"date-parts":[[2020,11,15]],"date-time":"2020-11-15T10:50:00Z","timestamp":1605437400000},"page":"2029-2041","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Morphological identification of thoracolumbar spinal afferent nerve endings in mouse uterus"],"prefix":"10.1002","volume":"529","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0555-868X","authenticated-orcid":false,"given":"Kelsi N.","family":"Dodds","sequence":"first","affiliation":[{"name":"College of Medicine and Public Health, Flinders Health and Medical Research Institute Flinders University of South Australia  Bedford Park South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5820-8432","authenticated-orcid":false,"given":"Melinda A.","family":"Kyloh","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health, Flinders Health and Medical Research Institute Flinders University of South Australia  Bedford Park South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1770-7486","authenticated-orcid":false,"given":"Lee","family":"Travis","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health, Flinders Health and Medical Research Institute Flinders University of South Australia  Bedford Park South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8256-0375","authenticated-orcid":false,"given":"Elizabeth A. H.","family":"Beckett","sequence":"additional","affiliation":[{"name":"Discipline of Physiology, Adelaide Medical School University of Adelaide  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2190-5303","authenticated-orcid":false,"given":"Nick J.","family":"Spencer","sequence":"additional","affiliation":[{"name":"College of Medicine and Public Health, Flinders Health and Medical Research Institute Flinders University of South Australia  Bedford Park South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.23434"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.09.028"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1988.59.1.142"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-27592-5_7"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.29"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410051211"},{"issue":"2","key":"e_1_2_10_8_1","first-page":"357","article-title":"Cholinergic, nitrergic and peptidergic (substance P\u2010 and CGRP\u2010utilizing) innervation of the horse intestine. 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