{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:35:13Z","timestamp":1790476513762,"version":"4.1.0"},"reference-count":92,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,8,22]],"date-time":"2021-08-22T00:00:00Z","timestamp":1629590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571324"],"award-info":[{"award-number":["81571324"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Orexin neurons play an important role in stress\u2010related mental disorders including post\u2010traumatic stress disorder (PTSD). Anxiety\u2010 and depression\u2010related symptoms commonly occur in combination with PTSD. However, the role of the orexin system in mediating alterations in these affective symptoms remains unclear. The medial prefrontal cortex (mPFC) is implicated in both cognitive and emotional processing. In the present study, we investigated anxiety\u2010 and depression\u2010related behavioral changes using the elevated plus maze, the sucrose preference test, and the open field test in male rats with single prolonged stress (SPS) induced\u2010PTSD. The expression of orexin\u2010A in the hypothalamus and orexin receptors (OX1R and OX2R) in the mPFC was detected and quantified by immunohistochemistry, western blotting, and real\u2010time polymerase chain reaction. We found that the SPS rats exhibited enhanced levels of anxiety, reduced exploratory activities, and anhedonia. Furthermore, SPS resulted in reductions in the expression of orexin\u2010A in the hypothalamus and the increased the expression of OX1R in the mPFC. The intracerebroventricular administration of orexin\u2010A alleviated behavioral changes in SPS rats and partly restored the increased levels of OX1R in the mPFC. These results suggest that the orexin system plays a role in the anxiety\u2010 and depression\u2010related symptoms observed in PTSD.<\/jats:p>","DOI":"10.1002\/cne.25231","type":"journal-article","created":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T10:07:20Z","timestamp":1628849240000},"page":"592-606","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["The effects of <scp>orexin\u2010A<\/scp> and orexin receptors on anxiety\u2010 and depression\u2010related behaviors in a male rat model of post\u2010traumatic stress disorder"],"prefix":"10.1002","volume":"530","author":[{"given":"Dan","family":"Han","sequence":"first","affiliation":[{"name":"PTSD Laboratory, Department of Histology and Embryology Basic Medical Sciences College, China Medical University  Shenyang China"},{"name":"Department of Neonatology The First Hospital of China Medical University  Shenyang 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