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While these projections have been studied in several mammals, a comprehensive examination of their organization in the mouse is lacking. We used the retrograde transport of viruses or cholera toxin subunit B (CTB) injected in the dLGN, immunocytochemical labeling with antibodies against choline acetyltransferase (ChAT), brain nitric oxide synthase (BNOS), and vesicular acetylcholine transporter (VAChT), ChAT\u2010Cre mice crossed with a reporter line (Ai9), as well as brainstem virus injections in ChAT\u2010Cre mice to examine the pattern of thalamic innervation from cholinergic neurons in the pedunculopontine tegmental nucleus (PPTg), laterodorsal tegmental nucleus (LDTg), and the parabigeminal nucleus (PBG). Retrograde tracing demonstrated that the dLGN receives input from the PPTg, LDTg, and PBG. Viral tracing in ChAT\u2010Cre mice and retrograde tracing combined with immunocytochemistry revealed that many of these inputs originate from cholinergic neurons in the PBG and PPTg. Most notable was an extensive cholinergic projection from the PBG which innervated most of the contralateral dLGN, with an especially dense concentration in the dorsolateral shell, as well as a small region in the dorsomedial pole of the ipsilateral dLGN. The PPTg was found to provide a sparse somewhat diffuse innervation of the ipsilateral dLGN. Neurons in the PPTg co\u2010expressed ChAT, BNOS, and VAChT, whereas PBG neurons expressed ChAT, but not BNOS or VAChT. These results highlight the presence of distinct cholinergic populations that innervate the mouse dLGN.<\/jats:p>","DOI":"10.1002\/cne.25235","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T03:51:28Z","timestamp":1630036288000},"page":"1081-1098","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["The organization of cholinergic projections in the visual thalamus of the mouse"],"prefix":"10.1002","volume":"530","author":[{"given":"Guela","family":"Sokhadze","sequence":"first","affiliation":[{"name":"Department of Anatomical Sciences and Neurobiology University of Louisville School of Medicine  Louisville Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle L.","family":"Whyland","sequence":"additional","affiliation":[{"name":"Department of Anatomical Sciences and Neurobiology University of Louisville School of Medicine  Louisville Kentucky 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