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During their dorsoventral migration, IO neurons extend long leading processes that cross the midline, transform into axons, and project into the contralateral cerebellum. In absence of the axon guidance receptor Robo3, IO axons fail to cross the midline and project to the ipsilateral cerebellum. Remarkably, the IO cell bodies still reach the midline where they form a nucleus of abnormal cytoarchitecture. The mechanisms underlying the migration of Robo3\u2010deficient IO neurons are unknown. Here, we used three\u2010dimensional imaging and transgenic mice to label subsets of IO neurons and study their migratory behavior in Robo3 knockout. We show that IO migration is delayed in absence of Robo3. Strikingly, Robo3\u2010deficient IO neurons progress toward the midline in a direction opposite to their axons. This occurs through a change of polarity and the generation of a second leading process at the rear of the cell. These results suggest that Robo3 receptor controls the establishment of neuronal polarity and the coupling of axonogenesis and cell body migration in IO neurons.<\/jats:p>","DOI":"10.1002\/cne.25381","type":"journal-article","created":{"date-parts":[[2022,7,10]],"date-time":"2022-07-10T23:39:52Z","timestamp":1657496392000},"page":"2868-2880","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Uncoupling axon guidance and neuronal migration in Robo3\u2010deficient inferior olivary neurons"],"prefix":"10.1002","volume":"530","author":[{"given":"Juan Antonio","family":"Moreno\u2010Bravo","sequence":"first","affiliation":[{"name":"Institut de la Vision Sorbonne Universit\u00e9, INSERM, CNRS Paris France"},{"name":"Instituto de Neurociencias de Alicante Universidad Miguel Hern\u00e1ndez\u2010Consejo Superior de Investigaciones Cient\u00edficas (UMH\u2010CSIC) San Juan de Alicante Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1501-0773","authenticated-orcid":false,"given":"Quentin","family":"Rappeneau","sequence":"additional","affiliation":[{"name":"Institut de la Vision Sorbonne Universit\u00e9, INSERM, CNRS Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergi","family":"Roig\u2010Puiggros","sequence":"additional","affiliation":[{"name":"Institut de la Vision Sorbonne Universit\u00e9, INSERM, CNRS Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constantino","family":"Sotelo","sequence":"additional","affiliation":[{"name":"Institut de la Vision Sorbonne Universit\u00e9, INSERM, CNRS Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7577-3794","authenticated-orcid":false,"given":"Alain","family":"Ch\u00e9dotal","sequence":"additional","affiliation":[{"name":"Institut de la Vision Sorbonne Universit\u00e9, INSERM, CNRS Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902570403"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902630402"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.03.018"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.03.008"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.10.037"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-11-04407.1999"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(88)90064-8"},{"key":"e_1_2_10_9_1","first-page":"151","article-title":"Early development of the rat precerebellar system: Migratory routes, selective aggregation and neuritic differentiation of the inferior olive and lateral reticular nucleus neurons. 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