{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:39:29Z","timestamp":1790818769310,"version":"4.1.0"},"reference-count":79,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T00:00:00Z","timestamp":1658016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The olfactory placode (OP) of vertebrates generates several classes of migrating cells, including hypothalamic gonadotropin\u2010releasing hormone (GnRH)\u2010producing neurons, which play essential roles in the reproduction system. Previous studies using OP cell labeling have demonstrated that OP\u2010derived non\u2010GnRH cells enter the developing forebrain; however, their final fates and phenotypes are less well understood. In chick embryos, a subpopulation of migratory cells from the OP that is distinct from GnRH neurons transiently expresses somatostatin (SS). We postulated that these cells are destined to develop into brain neurons. In this study, we examined the expression pattern of SS mRNA in the olfactory\u2010forebrain region during development, as well as the destination of OP\u2010derived migratory cells, including SS mRNA\u2010expressing cells. Utilizing the Tol2 genomic integration system to induce long\u2010term fluorescent protein expression in OP cells, we found that OP\u2010derived migratory cells labeled at embryonic day (E) 3 resided in the olfactory nerve and medial forebrain at E17\u201319. A subpopulation of green fluorescent protein (GFP)\u2010labeled GnRH neurons that remained in the olfactory nerve was considered to comprise terminal nerve neurons. In the forebrain, GFP\u2010labeled cells showed a distribution pattern similar to that of GnRH neurons. A large proportion of GFP\u2010labeled cells expressed the mature neuronal marker NeuN. Among the GFP\u2010labeled cells, the percentage of GnRH neurons was low, while the remaining GnRH\u2010negative neurons either expressed SS mRNA, neuropeptide Y, or calbindin D\u201028k or did not express any of them. These results indicate that a diverse population of OP\u2010derived neuronal cells, other than GnRH neurons, integrates into the chick medial forebrain.<\/jats:p>","DOI":"10.1002\/cne.25389","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T00:19:55Z","timestamp":1658103595000},"page":"2977-2993","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Olfactory placode generates a diverse population of neurons expressing GnRH, somatostatin mRNA, neuropeptide Y, or calbindin in the chick forebrain"],"prefix":"10.1002","volume":"530","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9813-1118","authenticated-orcid":false,"given":"Shizuko","family":"Murakami","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Neuropathology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Ohki\u2010Hamazaki","sequence":"additional","affiliation":[{"name":"College of Liberal Arts and Science Kitasato University  Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9104-533X","authenticated-orcid":false,"given":"Yasuo","family":"Uchiyama","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Neuropathology Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.32041"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(92)90382-H"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.4.1488"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90081-I"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402320304"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01258490"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20372"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90091-H"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(85)90260-X"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(95)00199-S"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20545"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199904)214:4<349::AID-AJA7>3.0.CO;2-E"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(86)90012-2"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1089"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00334-7"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2003.07.004"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2014.09.004"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6087-10.2011"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22164"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1050880104"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199612)31:4<487::AID-NEU8>3.0.CO;2-5"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.1197"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00537-X"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/166.2.895"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2005.12.006"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00319038"},{"key":"e_1_2_8_28_1","volume-title":"A stereotaxic atlas of the brain of the chick (Gallus domesticus)","author":"Kuenzel W. J.","year":"1988"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21461"},{"key":"e_1_2_8_30_1","first-page":"47","article-title":"Cell migration from the chick olfactory placode: A light and electron microscopic study","volume":"69","author":"Mendoza A. S.","year":"1982","journal-title":"Journal of Embryology and Experimental Morphology"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22696"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402320305"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22497"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.14.489"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.116.1.201"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.1.6425"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-0102(94)90046-9"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(94)90169-4"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22319"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(20000501)420:2<171::AID-CNE2>3.0.CO;2-9"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-0102(91)90073-8"},{"key":"e_1_2_8_42_1","first-page":"85","article-title":"Determination of the embryonic origin of the mesencephalic nucleus of the trigeminal nerve in birds","volume":"43","author":"Narayanan C. H.","year":"1978","journal-title":"Journal of Embryology and Experimental Morphology"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1993.1314"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(92)90436-B"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2019.09.003"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(94)90090-6"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2016-1363"},{"key":"e_1_2_8_48_1","volume-title":"The chick brain in stereotaxic coordinates","author":"Puelles L.","year":"2007"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-012-0486-2"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90525-7"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(81)91054-4"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.32.18923"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.01.043"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/338161a0"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10387"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-0102(09)80003-5"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00035"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0007-1331.2001.00762.x"},{"key":"e_1_2_8_59_1","first-page":"1683","volume-title":"The physiology of reproduction","author":"Silverman A. J.","year":"1994"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(91)90489-P"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1159\/000126598"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902020109"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.601923"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.12.367"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2826.2001.00685.x"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.137.12.8940365"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.106.060244"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(93)90173-8"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(96)00147-2"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/ca.22130"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2007.09.007"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20095"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-007-0432-4"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10655"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20094"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)91655-Z"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(02)41083-7"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2010.02034.x"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.20.8132"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(96)00078-8"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.25389","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cne.25389","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.25389","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T23:48:44Z","timestamp":1692661724000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.25389"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,17]]},"references-count":79,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/cne.25389"],"URL":"https:\/\/doi.org\/10.1002\/cne.25389","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/CNE.25389\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25389\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25389\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25389\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25389\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25389\/v1\/review2","asserted-by":"object"}]},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,17]]},"assertion":[{"value":"2021-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}