{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T12:59:27Z","timestamp":1789131567456,"version":"build-2803163510"},"reference-count":74,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS099425"],"award-info":[{"award-number":["NS099425"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Neuropeptide S (NPS) increases wakefulness. A small number of neurons in the brainstem express\n                    <jats:italic>Nps<\/jats:italic>\n                    . These neurons are located in or near the parabrachial nucleus (PB), but we know very little about their ontogeny, connectivity, and function. To identify\n                    <jats:italic>Nps<\/jats:italic>\n                    \u2010expressing neurons within the molecular framework of the PB region, we used in situ hybridization, immunofluorescence, and Cre\u2010reporter labeling in mice. The primary concentration of\n                    <jats:italic>Nps<\/jats:italic>\n                    \u2010expressing neurons borders the lateral lemniscus at far\u2010rostral levels of the lateral PB. Caudal to this main cluster,\n                    <jats:italic>Nps<\/jats:italic>\n                    \u2010expressing neurons scatter through the PB and form a secondary concentration medial to the locus coeruleus (LC). Most\n                    <jats:italic>Nps<\/jats:italic>\n                    \u2010expressing neurons in the PB region are\n                    <jats:italic>Atoh1<\/jats:italic>\n                    \u2010derived,\n                    <jats:italic>Foxp2<\/jats:italic>\n                    \u2010expressing, and mutually exclusive with neurons expressing\n                    <jats:italic>Calca<\/jats:italic>\n                    or\n                    <jats:italic>Lmx1b<\/jats:italic>\n                    . Among\n                    <jats:italic>Foxp2<\/jats:italic>\n                    \u2010expressing PB neurons, those expressing\n                    <jats:italic>Nps<\/jats:italic>\n                    are distinct from intermingled subsets expressing\n                    <jats:italic>Cck<\/jats:italic>\n                    or\n                    <jats:italic>Pdyn<\/jats:italic>\n                    . Examining\n                    <jats:italic>Nps<\/jats:italic>\n                    Cre\u2010reporter expression throughout the brain identified novel populations of neurons in the nucleus incertus, anterior hypothalamus, and lateral habenula. This information will help focus experimental questions about the connectivity and function of NPS neurons.\n                  <\/jats:p>","DOI":"10.1002\/cne.25400","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T06:14:31Z","timestamp":1661753671000},"page":"3157-3178","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Neuropeptide S (NPS) neurons: Parabrachial identity and novel distributions"],"prefix":"10.1002","volume":"530","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2063-8995","authenticated-orcid":false,"given":"Dake","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Neurology University of Iowa  Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5874-3734","authenticated-orcid":false,"given":"Richie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Iowa  Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Gasparini","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Iowa  Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam C.","family":"McDonough","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Pharmacology University of Iowa  Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Paradee","sequence":"additional","affiliation":[{"name":"Genome Editing Core Facility University of Iowa  Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9956-4006","authenticated-orcid":false,"given":"Joel C.","family":"Geerling","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Iowa  Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2015.00126"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429\u2010015\u20101117\u20105"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.15.8730"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201017204\u20105"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/36579"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22698"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1037\/h0022308"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109384(84)90323\u20108"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-03-00613.1986"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22606"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/mp.2010.81"},{"key":"e_1_2_11_13_1","volume-title":"Allen reference atlas: A digital color brain atlas of the C57Black\/6J male mouse","author":"Dong H. W.","year":"2008"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2010.05.039"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726\u2010011\u20101058\u20100"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2017.04.005"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20370"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0173(84)90012-2"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2014.11.006"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00261.2020"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429\u2010018\u20101778\u2010y"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00094.2015"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20993"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21452"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.11.028"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24164"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.07.031"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1303"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102350\u20108\u2010S1\u2010S9"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhaa072"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01383.2007"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10221"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2020.03.011"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0091\u20103057(01)00681\u20105"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.073"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24975"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.25136"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.56605"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21891"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.25307"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593\u2010018\u20100204\u20103"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1096\u20109861(19980427)394:148::aid\u2010cne53.0.co;2\u2010h"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12956"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090010"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101053\u20102"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.02.028"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22603"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(79)90584-5"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201014116\u2010y"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)00481-J"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.08.028"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05860"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2012.05.049"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0149\u20107634(94)90023\u2010x"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.153"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2007.06.026"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2018.03.007"},{"key":"e_1_2_11_58_1","volume-title":"Paxinos and Franklin's: The mouse brain in stereotaxic coordinates","author":"Paxinos G.","year":"2013"},{"key":"e_1_2_11_59_1","volume-title":"The rat brain in stereotaxic coordinates","author":"Paxinos G.","year":"2007"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-057480-6.50005-8"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.093427"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911579106"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2011.05.003"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005\u20101280"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098789"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3145"},{"key":"e_1_2_11_67_1","volume-title":"Brain maps: Structure of the rat brain","author":"Swanson L. W.","year":"1992"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abd6469"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.07.009"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24215"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23812"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2005.08.024"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aax2014"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21159"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.08.005"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.25400","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cne.25400","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.25400","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T04:16:47Z","timestamp":1692591407000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.25400"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,29]]},"references-count":74,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/cne.25400"],"URL":"https:\/\/doi.org\/10.1002\/cne.25400","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/CNE.25400\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25400\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25400\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25400\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25400\/v1\/review1","asserted-by":"object"}]},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,29]]},"assertion":[{"value":"2022-05-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}