{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T02:09:36Z","timestamp":1773972576838,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cholesterol\u201024\u2010hydroxylase (CYP46), a member of the\n                    <jats:italic>cytochrome P450<\/jats:italic>\n                    superfamily of enzymes, is selectively expressed in the brain and is mainly responsible for cholesterol turnover in the central nervous system. Although increased\n                    <jats:italic>cyp46A1<\/jats:italic>\n                    gene expression has been linked to cognitive alterations in aging and observed in neurodegenerative diseases and after traumatic brain injury, a detailed characterization of the brain regions and cell types in which CYP46 is expressed in old individuals has not been performed. Using immunohistochemistry and immunofluorescence, we investigated the specific regions and cell populations in the brain, in which\n                    <jats:italic>cyp46A1<\/jats:italic>\n                    is expressed in 24\u2010month\u2010old mice. We found that CYP46 is localized in the same neuronal populations in young and old brains, mainly in the hippocampus, in cortical layers, and in Purkinje neurons of the cerebellum. No increase in CYP46 levels was found in astrocytes in old mice brains, in primary astrocyte\u2010neuron cocultures aged in vitro, or in primary cultures of senescent astrocytes. However, interleukin\u20106 treatment strongly induced\n                    <jats:italic>cyp46A1<\/jats:italic>\n                    expression in reactive astrocytes characterized by high GFAP levels but had no effect in nonactivated astrocytes. Our data suggest that cholesterol\u201024\u2010hydroxylase expression is triggered in reactive astrocytes in response to proinflammatory signals, probably as part of a response mechanism to injury.\n                  <\/jats:p>","DOI":"10.1002\/cne.25436","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T08:27:08Z","timestamp":1669883228000},"page":"486-499","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cholesterol\u201024\u2010hydroxylase (CYP46) in the old brain: Analysis of positive populations and factors triggering its expression in astrocytes"],"prefix":"10.1002","volume":"531","author":[{"given":"Gabriel G.","family":"Cataldi","sequence":"first","affiliation":[{"name":"Instituto Ferreyra, INIMEC\u2010CONICET\u2010UNC Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Setiembre D.","family":"Elorza","sequence":"additional","affiliation":[{"name":"Instituto Ferreyra, INIMEC\u2010CONICET\u2010UNC Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9311-6226","authenticated-orcid":false,"given":"Ana","family":"Toledano\u2010Zaragoza","sequence":"additional","affiliation":[{"name":"Molecular Neuropathology Unit, Physiological and Pathological Processes Program Centro de Biolog\u00eda Molecular Severo Ochoa (CBMSO\u2010CSIC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soledad","family":"de Olmos","sequence":"additional","affiliation":[{"name":"Instituto Ferreyra, INIMEC\u2010CONICET\u2010UNC Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea B.","family":"Cragnolini","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones 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