{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:13:35Z","timestamp":1790190815034,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T00:00:00Z","timestamp":1670371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008431","name":"Consejer\u00eda de Educaci\u00f3n, Junta de Castilla y Le\u00f3n","doi-asserted-by":"publisher","award":["SA136G18"],"award-info":[{"award-number":["SA136G18"]}],"id":[{"id":"10.13039\/501100008431","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Oligodendrocytes are the myelinating cells in the central nervous system. In birds and mammals, the oligodendrocyte progenitor cells (OPCs) originate in the preoptic area (POA) of the hypothalamus. However, it remains unclear in other vertebrates such as fish. Thus, we have studied the early progression of OPCs during zebrafish visual morphogenesis from 2 days post fertilization (dpf) until 11 dpf using the\n                    <jats:italic>olig2:EGFP<\/jats:italic>\n                    transgenic line; and we have analyzed the differential expression of transcription factors involved in oligodendrocyte differentiation: Sox2 (using immunohistochemistry) and Sox10 (using the transgenic line\n                    <jats:italic>sox10:tagRFP<\/jats:italic>\n                    ). The first OPCs (\n                    <jats:italic>olig2:EGFP<\/jats:italic>\n                    \/Sox2) were found at 2 dpf in the POA. From 3 dpf onwards, these\n                    <jats:italic>olig2:EGFP<\/jats:italic>\n                    \/Sox2 cells migrate to the optic chiasm, where they invade the optic nerve (ON), extending toward the retina. At 5 dpf,\n                    <jats:italic>olig2:EGFP<\/jats:italic>\n                    \/Sox2 cells in the ON also colocalize with\n                    <jats:italic>sox10:tagRFP<\/jats:italic>\n                    . When\n                    <jats:italic>olig2:EGFP<\/jats:italic>\n                    cells differentiate and present more projections, they become positive only for\n                    <jats:italic>sox10:tagRFP<\/jats:italic>\n                    .\n                    <jats:italic>olig2:EGFP\/sox10: tagRFP<\/jats:italic>\n                    cells ensheath the ON by 5 dpf when they also become positive for a myelin marker, based on the\n                    <jats:italic>mbpa:tagRFPt<\/jats:italic>\n                    transgenic line. We also found\n                    <jats:italic>olig2:EGFP<\/jats:italic>\n                    cells in other regions of the visual system. In the central retina at 2 dpf, they are positive for Sox2 but later become restricted to the proliferative germinal zone without this marker. In the ventricular areas of the optic tectum,\n                    <jats:italic>olig2:EGFP<\/jats:italic>\n                    cells present Sox2 but arborized ones\n                    <jats:italic>sox10:tagRFP<\/jats:italic>\n                    instead. Our data matches with other models, where OPCs are specified in the POA and migrate to the ON through the optic chiasm.\n                  <\/jats:p>","DOI":"10.1002\/cne.25440","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T17:12:26Z","timestamp":1670433146000},"page":"515-527","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Oligodendrocyte origin and development in the zebrafish visual system"],"prefix":"10.1002","volume":"531","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6814-0718","authenticated-orcid":false,"given":"Adri\u00e1n","family":"Santos\u2010Ledo","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Pathology, Instituto de NeurocienciasdeCastilla y Le\u00f3n (INCyL) Universidad de Salamanca  Salamanca Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de Salamanca (IBSAL)  Salamanca 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Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5849-1736","authenticated-orcid":false,"given":"Rosario","family":"Ar\u00e9valo","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Pathology, Instituto de NeurocienciasdeCastilla y Le\u00f3n (INCyL) Universidad de Salamanca  Salamanca Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de Salamanca (IBSAL)  Salamanca Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7442-3255","authenticated-orcid":false,"given":"Almudena","family":"Velasco","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Pathology, Instituto de NeurocienciasdeCastilla y Le\u00f3n (INCyL) Universidad de Salamanca  Salamanca Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica de Salamanca (IBSAL)  Salamanca 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