{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T07:41:18Z","timestamp":1782632478865,"version":"3.54.5"},"reference-count":81,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T00:00:00Z","timestamp":1681862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1186915"],"award-info":[{"award-number":["1186915"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study sought to identify demographic variations in retinal thickness measurements from optical coherence tomography (OCT), to enable the calculation of cell density parameters across the neural layers of the healthy human macula. From macular OCTs (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0247), ganglion cell (GCL), inner nuclear (INL), and inner segment\u2013outer segment (ISOS) layer measurements were extracted using a customized high\u2010density grid. Variations with age, sex, ethnicity, and refractive error were assessed with multiple linear regression analyses, with age\u2010related distributions further assessed using hierarchical cluster analysis and regression models. Models were tested on a na\u00efve healthy cohort (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a040) with Mann\u2013Whitney tests to determine generalizability. Quantitative cell density data were calculated from histological data from previous human studies. Eccentricity\u2010dependent variations in OCT retinal thickness closely resemble topographic cell density maps from human histological studies. Age was consistently identified as significantly impacting retinal thickness (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a0.0006, .0007, and .003 for GCL, INL and ISOS), with gender affecting ISOS only (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a0.0001). Regression models demonstrated that age\u2010related changes in the GCL and INL begin in the 30th decade and were linear for the ISOS. Model testing revealed significant differences in INL and ISOS thickness (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a0.0008 and .0001; however, differences fell within the OCT's axial resolution. Qualitative comparisons show close alignment between OCT and histological cell densities when using unique, high\u2010resolution OCT data, and correction for demographics\u2010related variability. Overall, this study describes a process to calculate in vivo cell density from OCT for all neural layers of the human retina, providing a framework for basic science and clinical investigations.\n                  <\/jats:p>","DOI":"10.1002\/cne.25483","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T03:11:49Z","timestamp":1681873909000},"page":"1108-1125","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Derivation of human retinal cell densities using high\u2010density, spatially localized optical coherence tomography data from the human retina"],"prefix":"10.1002","volume":"531","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4268-5138","authenticated-orcid":false,"given":"Janelle","family":"Tong","sequence":"first","affiliation":[{"name":"Centre for Eye Health University of New South Wales (UNSW) New South Wales Sydney Australia"},{"name":"School of Optometry and Vision Science University of New South Wales (UNSW) New South Wales Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4696-9226","authenticated-orcid":false,"given":"Vincent","family":"Khou","sequence":"additional","affiliation":[{"name":"Centre for Eye Health University of New South Wales (UNSW) New South Wales Sydney Australia"},{"name":"School of Optometry and Vision Science University of New South Wales (UNSW) New South Wales Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt","family":"Trinh","sequence":"additional","affiliation":[{"name":"Centre for Eye Health University of New South Wales (UNSW) New South Wales Sydney Australia"},{"name":"School of Optometry and Vision Science University of New South Wales (UNSW) New South Wales Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Alonso\u2010Caneiro","sequence":"additional","affiliation":[{"name":"School of Optometry and Vision Science Centre for Vision and Eye Research Contact Lens and Visual Optics Laboratory Queensland University of Technology Queensland Brisbane Australia"},{"name":"School of Science, Technology and Engineering University of Sunshine Coast Queensland Sippy Downs Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Zangerl","sequence":"additional","affiliation":[{"name":"School of Optometry and Vision Science University of New South Wales (UNSW) New South Wales Sydney Australia"},{"name":"Coronary Care Unit Royal Prince Alfred Hospital New South Wales Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5264-4639","authenticated-orcid":false,"given":"Michael","family":"Kalloniatis","sequence":"additional","affiliation":[{"name":"Centre for Eye Health University of New South Wales (UNSW) New South Wales Sydney Australia"},{"name":"School of Optometry and Vision Science University of New South Wales (UNSW) New South Wales Sydney Australia"},{"name":"Department of Optometry School of Medicine Deakin University Victoria Waurn Ponds Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-007-0029-9"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.7.000629"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.62.1.5"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/2055217319871582"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1068\/p010371"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-25332"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annepidem.2006.05.007"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7723706"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-55484-1"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903000103"},{"key":"e_1_2_11_12_1","first-page":"1236","article-title":"Photoreceptor loss in age\u2010related macular degeneration","volume":"37","author":"Curcio C. 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