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Peripheral nociceptive afferents can be detected with a variety of markers (e.g., substance P [SP] and calcitonin gene\u2010related peptide [CGRP]). We recently examined the topographical organization and morphology of SP\u2010immunoreactive (SP\u2010IR) axons in the whole mouse stomach muscular layer. However, the distribution and morphological structure of CGRP\u2010IR axons remain unclear. We used immunohistochemistry labeling and applied a combination of imaging techniques, including confocal and Zeiss Imager M2 microscopy, Neurolucida 360 tracing, and integration of axon tracing data into a 3D stomach scaffold to characterize CGRP\u2010IR axons and terminals in the whole mouse stomach muscular layers. We found that: (1) CGRP\u2010IR axons formed extensive terminal networks in both ventral and dorsal stomachs. (2) CGRP\u2010IR axons densely innervated the blood vessels. (3) CGRP\u2010IR axons ran in parallel with the longitudinal and circular muscles. Some axons ran at angles through the muscular layers. (4) They also formed varicose terminal contacts with individual myenteric ganglion neurons. (5) CGRP\u2010IR occurred in DiI\u2010labeled gastric\u2010projecting neurons in the dorsal root and vagal nodose ganglia, indicating CGRP\u2010IR axons were visceral afferent axons. (6) CGRP\u2010IR axons did not colocalize with tyrosine hydroxylase or vesicular acetylcholine transporter axons in the stomach, indicating CGRP\u2010IR axons were not visceral efferent axons. (7) CGRP\u2010IR axons were traced and integrated into a 3D stomach scaffold. For the first time, we provided a topographical distribution map of CGRP\u2010IR axon innervation of the whole stomach muscular layers at the cellular\/axonal\/varicosity scale.<\/jats:p>","DOI":"10.1002\/cne.25519","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T07:10:21Z","timestamp":1694416221000},"page":"1608-1632","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Organization and morphology of calcitonin gene\u2010related peptide\u2010immunoreactive axons in the whole mouse stomach"],"prefix":"10.1002","volume":"531","author":[{"given":"Jichao","family":"Ma","sequence":"first","affiliation":[{"name":"Burnett School of Biomedical Sciences, College of Medicine University of Central Florida Orlando Florida 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