{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T11:39:45Z","timestamp":1791805185821,"version":"4.3.4"},"reference-count":97,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T00:00:00Z","timestamp":1696204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001201","name":"Kavli Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001201","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Accurate anatomical characterizations are necessary to investigate neural circuitry on a fine scale, but for the rodent claustrum complex (CLCX), this has yet to be fully accomplished. The CLCX is generally considered to comprise two major subdivisions, the claustrum (CL) and the dorsal endopiriform nucleus (DEn), but regional boundaries to these areas are debated. To address this, we conducted a multifaceted analysis of fiber\u2010 and cytoarchitecture, genetic marker expression, and connectivity using mice of both sexes, to create a comprehensive guide for identifying and delineating borders to CLCX, including an online reference atlas. Our data indicated four distinct subregions within CLCX, subdividing both CL and DEn into two. Additionally, we conducted brain\u2010wide tracing of inputs to CLCX using a transgenic mouse line. Immunohistochemical staining against myelin basic protein (MBP), parvalbumin (PV), and calbindin (CB) revealed intricate fiber\u2010architectural patterns enabling precise delineations of CLCX and its subregions. Myelinated fibers were abundant dorsally in CL but absent ventrally, whereas PV expressing fibers occupied the entire CL. CB staining revealed a central gap within CL, also visible anterior to the striatum. The\n                    <jats:italic>Nr2f2, Npsr1<\/jats:italic>\n                    , and\n                    <jats:italic>Cplx3<\/jats:italic>\n                    genes expressed specifically within different subregions of the CLCX, and\n                    <jats:italic>Rprm<\/jats:italic>\n                    helped delineate the CL\u2010insular border. Furthermore, cells in CL projecting to the retrosplenial cortex were located within the myelin sparse area. By combining own experimental data with digitally available datasets of gene expression and input connectivity, we could demonstrate that the proposed delineation scheme allows anchoring of datasets from different origins to a common reference framework.\n                  <\/jats:p>","DOI":"10.1002\/cne.25539","type":"journal-article","created":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T14:05:14Z","timestamp":1696255514000},"page":"1772-1795","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["A multifaceted architectural framework of the mouse claustrum complex"],"prefix":"10.1002","volume":"531","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7011-0721","authenticated-orcid":false,"given":"Joachim S.","family":"Grimstvedt","sequence":"first","affiliation":[{"name":"Kavli Institute for Systems Neuroscience NTNU Norwegian University of Science and Technology Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5787-4310","authenticated-orcid":false,"given":"Andrew M.","family":"Shelton","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy &amp; Genetics University of Oxford Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1953-7871","authenticated-orcid":false,"given":"Anna","family":"Hoerder\u2010Suabedissen","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy &amp; Genetics University of Oxford Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1210-8409","authenticated-orcid":false,"given":"David K.","family":"Oliver","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy &amp; Genetics University of Oxford Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christin H.","family":"Berndtsson","sequence":"additional","affiliation":[{"name":"Kavli Institute for Systems Neuroscience NTNU Norwegian University of Science and Technology Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0993-3790","authenticated-orcid":false,"given":"Stefan","family":"Blankvoort","sequence":"additional","affiliation":[{"name":"Kavli Institute for Systems Neuroscience NTNU Norwegian University of Science and Technology Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9755-0874","authenticated-orcid":false,"given":"Rajeevkumar R.","family":"Nair","sequence":"additional","affiliation":[{"name":"Kavli Institute for Systems Neuroscience NTNU Norwegian University of Science and Technology Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5884-794X","authenticated-orcid":false,"given":"Adam M.","family":"Packer","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy &amp; Genetics University of Oxford Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0285-1637","authenticated-orcid":false,"given":"Menno P.","family":"Witter","sequence":"additional","affiliation":[{"name":"Kavli Institute for Systems Neuroscience NTNU Norwegian University of Science and Technology Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3850-3829","authenticated-orcid":false,"given":"Clifford G.","family":"Kentros","sequence":"additional","affiliation":[{"name":"Kavli Institute for Systems Neuroscience NTNU Norwegian University of Science and Technology Trondheim Norway"},{"name":"Institute of Neuroscience University of Oregon Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00191452"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22073"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10875"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14\u201004\u201002020.1994"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000080243"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23997"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.06.068"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24110"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.931930"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhs038"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096\u20109861(19990614)408:4<532::AID\u2010CNE7>3.0.CO;2\u2010S"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201015390\u20105"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2019.00034"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.05.015"},{"key":"e_1_2_10_16_1","doi-asserted-by":"crossref","unstructured":"Bogovic J. A. Hanslovsky P. Wong A. &Saalfeld S.(2016).Robust registration of calcium images by learned contrast synthesis. In2016 IEEE 13th International Symposium on Biomedical Imaging (ISBI)(pp.1123\u20131126).IEEE.https:\/\/doi.org\/10.1109\/Isbi.2016.7493463","DOI":"10.1109\/ISBI.2016.7493463"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064\u2010019\u201002822\u20104"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24922"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749\u20106632.2011.06022.x"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1267"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(90)90091\u2010H"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22606"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnsys.2014.00042"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2005.1661"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20347"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891\u20100618(97)10013\u20108"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/2398212817718962"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2019.00053"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0891\u20100618(93)90014\u2010U"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2014.00160"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.68967"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165\u20100270(96)02204\u20102"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2021.786329"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429\u2010016\u20101290\u20101"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429\u2010017\u20101585\u2010x"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnsys.2014.00063"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2015.05.006"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/7.4.347"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0216\u201020.2020"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2013.01.014"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2022.901807"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460\u20109568.1994.tb00592.x"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306\u20104522(03)00100\u20103"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhac318"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891\u20100618(98)00065\u20109"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.14280"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.25349"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098\u20101063(1997)7:2<146::AID\u2010HIPO4>3.0.CO;2\u2010L"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2015.00250"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1360\u201016.2016"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1159\/000006581"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05453"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2021.632549"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10346"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-369497-3.10024-X"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.25043"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhn253"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20141"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429\u2010022\u201002459\u20100"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593\u2010020\u20100625\u20107"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/288479a0"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24036"},{"key":"e_1_2_10_63_1","volume-title":"The mouse brain in stereotaxic coordinates","author":"Paxinos G.","year":"2019"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2019.00096"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.13302"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2015.02.004"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12706"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2018.00100"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.05.011"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891\u20100618(02)00104\u20107"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050211"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24673"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2015.03.005"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1101\/2022.05.06.490864"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4438\u201010.2010"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24537"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1524\u201012.2012"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22295"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.06.033"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429\u2010010\u20100247\u2010z"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24047"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112118"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnsys.2014.00098"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-369497-3.10021-4"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-369497-3.10025-1"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24034"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23981"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2442\u201010.2010"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23970"},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-369497-3.10005-6"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012547638-6\/50022-5"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306\u20104522(88)90347\u20108"},{"issue":"1","key":"e_1_2_10_94_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.55782\/ane-2006-1585","article-title":"The anatomical relationships between the serotonergic afferents and the neurons containing calcium\u2010binding proteins in the rat claustrum","volume":"66","author":"Wojcik S.","year":"2006","journal-title":"Acta Neurobiologiae Experimentalis (Wars)"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306\u20104522(02)00084\u20102"},{"key":"e_1_2_10_96_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01941033"},{"key":"e_1_2_10_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-369497-3.10019-6"},{"key":"e_1_2_10_98_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24502"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.25539","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,23]],"date-time":"2023-12-23T01:46:04Z","timestamp":1703295964000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.25539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,2]]},"references-count":97,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1002\/cne.25539"],"URL":"https:\/\/doi.org\/10.1002\/cne.25539","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/CNE.25539\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25539\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25539\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25539\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25539\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25539\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25539\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25539\/v2\/response1","asserted-by":"object"}]},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,2]]},"assertion":[{"value":"2023-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}