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They are also suggested to play a role in efferent regulation because of secretory vesicles in the axoplasm. In the present study, the laminar nerve endings in the rat laryngeal mucosa were analyzed by 3D reconstruction from serial ultrathin sections in addition to immunohistochemistry for synapsin 1. In the light microscopy, synapsin 1\u2010immunoreactive flattened or bulbous terminal parts of the laminar endings were also immunoreactive with VGLUT1, and were surrounded by S100\u2010 or S100B\u2010immunoreactive Schwann cells and vimentin\u2010immunoreactive fibroblasts. In the electron microscopy, 3D reconstruction views showed that laminar endings were composed of flattened terminal parts sized 2\u20135\u00a0\u03bcm in longitudinal length, overlapping in three to five multiple layers. The terminal parts of the endings were incompletely wrapped by flat cytoplasmic processes of the Schwann cells. In addition, the fibroblast network surrounded the complex of nerve endings and the Schwann cells. Several terminal parts entered through the basement membrane into the epithelial layer and attached to the basal epithelial cells, suggesting that interaction between epithelial cells and laminar nerve endings plays an important role in sensing the pressure changes in the laryngeal cavity. Secretory vesicles were unevenly distributed throughout the terminal part of the laminar nerve endings. The secretory vesicles were frequently observed in the peripheral limb of the terminal parts. It suggests that the laminar nerve endings in the larynx may release glutamate to maintain continuous discharge during the stretching of the laryngeal mucosa.<\/jats:p>","DOI":"10.1002\/cne.25544","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T19:30:53Z","timestamp":1696534253000},"page":"1846-1866","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Three\u2010dimensional architecture of the subepithelial corpuscular nerve ending in the rat epiglottis reconstructed by array tomography with scanning electron microscopy"],"prefix":"10.1002","volume":"531","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6352-6013","authenticated-orcid":false,"given":"Yoshio","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture Iwate University  Morioka 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