{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:22:17Z","timestamp":1791861737035,"version":"4.3.4"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T00:00:00Z","timestamp":1708387200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["EY012793"],"award-info":[{"award-number":["EY012793"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Intrinsically photosensitive retinal ganglion cells (ipRGCs) are specialized retinal output neurons that mediate behavioral, neuroendocrine, and developmental responses to environmental light. There are diverse molecular strategies for marking ipRGCs, especially in mice, making them among the best characterized retinal ganglion cells (RGCs). With the development of more sensitive reporters, new subtypes of ipRGCs have emerged. We therefore tested high\u2010sensitivity reporter systems to see whether we could reveal yet more. Substantial confusion remains about which of the available methods, if any, label all and only ipRGCs. Here, we compared many different methods for labeling of ipRGCs, including anti\u2010melanopsin immunofluorescence, Opn4\u2010GFP BAC transgenic mice, and Opn4\n                    <jats:sup>cre<\/jats:sup>\n                    mice crossed with three different Cre\u2010specific reporters (Z\/EG, Ai9, and Ai14) or injected with Cre\u2010dependent (DIO) AAV2. We show that Opn4\n                    <jats:sup>cre<\/jats:sup>\n                    mice, when crossed with sensitive Cre\u2010reporter mice, label numerous ganglion cell types that lack intrinsic photosensitivity. Though other methods label ipRGCs specifically, they do not label the entire population of ipRGCs. We conclude that no existing method labels all and only ipRGCs. We assess the appropriateness of each reporter for particular applications and integrate findings across reporters to estimate that the overall abundance of ipRGCs among mouse RGCs may approach 11%.\n                  <\/jats:p>","DOI":"10.1002\/cne.25591","type":"journal-article","created":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T04:26:46Z","timestamp":1708403206000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Efficacy and specificity of melanopsin reporters for retinal ganglion cells"],"prefix":"10.1002","volume":"532","author":[{"given":"Ryan","family":"Maloney","sequence":"first","affiliation":[{"name":"Department of Neuroscience Brown University  Providence Rhode Island USA"},{"name":"Department of Organismic and Evolutionary Biology Harvard University  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Quattrochi","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Brown University  Providence Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Brown University  Providence Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Brown University  Providence Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Berson","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Brown University  Providence Rhode Island 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