{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T20:13:41Z","timestamp":1781036021039,"version":"3.54.1"},"reference-count":61,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The flower\u2010spray nerve endings are afferent nerve terminals in the carotid sinus that arise from carotid sinus nerve of glossopharyngeal nerve. However, the three\u2010dimensional ultrastructural characteristics of flower\u2010spray nerve endings and spatial relationships between the terminal parts and other cellular elements have not been fully understood. To elucidate their detailed relationship, backscattered electron imaging of serial sections was performed with a scanning electron microscope to produce a three\u2010dimensional reconstruction of the flower\u2010spray endings. The terminal parts of flower\u2010spray endings were distributed horizontally approximately 5\u00a0\u00b5m outside the external elastic membrane in the tunica adventitia of the internal carotid artery. The three\u2010dimensional reconstruction showed that the terminal parts of flower\u2010spray endings were flat with irregular contours and were partially covered by the thin cytoplasmic processes of Schwann cells. The complex consisting of the nerve terminals and associated Schwann cells was surrounded by a multilayered basement membrane. The terminal parts of the endings were also surrounded by fibroblasts with elastic fibers and collagen fibrils. Secretory vesicles without an electron\u2010dense core were observed in the terminal parts of the endings. The accumulation of vesicles just below the axonal membrane was observed in terminal parts not covered by Schwann cell cytoplasmic processes on both the luminal and basal sides. Swollen mitochondria, concentric membranous structures, and glycogen granule\u2010like electron\u2010dense materials were often noted in some of the terminal parts of the endings and the parent axon. Collectively, the present results suggest that flower\u2010spray endings are baroreceptors because their morphology was similar to other mechanoreceptors. Furthermore, flower\u2010spray endings may be affected by glutamate secreted in an autocrine manner.<\/jats:p>","DOI":"10.1002\/cne.25654","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T09:31:12Z","timestamp":1720517472000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Three\u2010Dimensional Ultrastructure of Flower\u2010Spray Nerve Endings in the Rat Carotid Sinus"],"prefix":"10.1002","volume":"532","author":[{"given":"Yusuke","family":"Murakami","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture Iwate University  Morioka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuniaki","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Center for Electron Microscopy Iwate University  Morioka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misaki","family":"Komuro","sequence":"additional","affiliation":[{"name":"Center for Electron Microscopy Iwate University  Morioka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3384-0623","authenticated-orcid":false,"given":"Takuya","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture Iwate University  Morioka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9876-2692","authenticated-orcid":false,"given":"Sayed Sharif","family":"Abdali","sequence":"additional","affiliation":[{"name":"Department of Anatomy (Cell Biology) Iwate Medical University  Yahaba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuaki","family":"Nakamuta","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture Iwate University  Morioka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6352-6013","authenticated-orcid":false,"given":"Yoshio","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture Iwate University  Morioka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361\u20109230(99)00233\u20106"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-65438-1_2"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.02.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.243659"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.074799"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00220113"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.NEUROSCIENCE.2009.07.015"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165\u20101838(97)00085\u20104"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096\u20109861(19970428)381:1"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.105097"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4065\/84.9.822"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/OXFORDJOURNALS.JMICRO.A049822"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102826.2011.02272.x"},{"key":"e_1_2_8_15_1","first-page":"331","article-title":"Sur la Structure et L'innervation Du Sinus Carotidien De L'homme et Des Mammif\u00e8res. Nouveaux Faits sur L'innervation et la Fonction Du Glomus Caroticum","volume":"25","author":"Castro F.","year":"1928","journal-title":"Travaux Du Laboratoire De Recherches Biologiques De L'universit\u00e9 De Madrid"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/NEURO.05.023.2009"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1098\/RSTB.2014.0191"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80661-3"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00525771"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.NEURON.2023.08.023"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2020.183408"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.25069"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.25219"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2015.00389"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155412464639"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.AUTNEU.2021.102860"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00338807\/METRICS"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00219267"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf01214800"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/NCOMMS11947"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395\u2010023\u201001011\u20103"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41598\u2010021\u201002563\u2010X"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.11.007"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.91.1.257"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411990100030401"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELREP.2019.10.040"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.AUTNEU.2022.103042"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01735375"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/AJPHEART.2001.281.1.H387"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/CNE.901310409"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2321447121"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/AR.1091790202"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418\u2010012\u20100939\u2010y"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302\u2010011\u20109249\u20104"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/JN.00315.2019"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19971208)389:1<177::AID-CNE13>3.0.CO;2-I"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096\u20109861(20001016)426:2<259::aid\u2010cne7>3.0.co;2\u2010n"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP281182"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0028\u20103908(97)00126\u20103"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673\u20105374.290911"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.0838\u201021.2021"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.57.473"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24351"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418\u2010021\u201002038\u20100"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/CNE.25544"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24779"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00232287"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00418\u2010018\u20101734\u20101"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ACTHIS.2019.151469"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24837"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00137.2003"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1126\/SCIENCE.AAU6324"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.25654","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T05:26:12Z","timestamp":1722317172000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.25654"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":61,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/cne.25654"],"URL":"https:\/\/doi.org\/10.1002\/cne.25654","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/CNE.25654\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25654\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25654\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25654\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25654\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25654\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25654\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/CNE.25654\/v3\/decision1","asserted-by":"object"}]},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7]]},"assertion":[{"value":"2024-01-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e25654"}}