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RNA\u2010sequencing (RNA\u2010Seq) was used to identify the expression of influx and efflux transporters (ATP\u2010binding cassettes [ABCs] and solute carriers [SLCs]) and metabolizing enzymes in brains of newborn to juvenile\n                    <jats:italic>Monodelphis<\/jats:italic>\n                    . Results were compared to published data in the developing eutherian rat. To test the functionality of these transporters at similar ages, the entry of paracetamol (acetaminophen) into the brain and cerebrospinal fluid (CSF) was measured using liquid scintillation counting following a single administration of the drug along with its radiolabelled tracer [\n                    <jats:sup>3<\/jats:sup>\n                    H]. Drug permeability studies found that in\n                    <jats:italic>Monodelphis<\/jats:italic>\n                    , brain entry of paracetamol was already restricted at P5; it decreased further in the first week of life and then remained stable until the oldest age group tested (P110). Transcriptomic analysis of\n                    <jats:italic>Monodelphis<\/jats:italic>\n                    brain showed that expression of transporters and their metabolizing enzymes in early postnatal (P) pups (P0, P5, and P8) was relatively similar, but by P109, many more transcripts were identified. When transcriptomes of newborn\n                    <jats:italic>Monodelphis<\/jats:italic>\n                    brain and E19 rat brain and placenta were compared, several transporters present in the rat placenta were also found in the newborn\n                    <jats:italic>Monodelphis<\/jats:italic>\n                    brain. These were absent from E19 rat brain but were present in the adult rat brain. These data indicate that despite its extreme immaturity, the newborn\n                    <jats:italic>Monodelphis<\/jats:italic>\n                    brain may compensate for the lack of placental protection during early brain development by upregulating protective mechanisms, which in eutherian animals are instead present in the placenta.\n                  <\/jats:p>","DOI":"10.1002\/cne.25655","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T08:55:17Z","timestamp":1720515317000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["ABC Efflux Transporters and Solute Carriers in the Early Developing Brain of a Marsupial\n                    <i>Monodelphis domestica<\/i>\n                    (South American Gray Short\u2010Tailed Opossum)"],"prefix":"10.1002","volume":"532","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1060-3668","authenticated-orcid":false,"given":"Yifan","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Neuroscience Monash University  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna M.","family":"Dziegielewska","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Monash University  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark D.","family":"Habgood","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Monash University  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Monash University  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C. 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