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However, as yet, there is no information on CART in the reptilian brain. We characterized the cDNA encoding CART and mapped CART\u2010containing elements in the brain of gecko, <jats:italic>Hemidactylus leschenaultii<\/jats:italic> (<jats:italic>hl<\/jats:italic>) using a specific anti\u2010CART antiserum. We report a 683\u2010bp <jats:italic>hlcart<\/jats:italic> transcript containing a 336\u2010bp open reading frame, which encodes a putative 111\u2010amino acid hl\u2010preproCART. The 89\u2010amino acid hl\u2010proCART generated from hl\u2010preproCART produced two putative bioactive hl\u2010CART\u2010peptides. These bioactive CART\u2010peptides were &gt;\u00a093% similar with those in rats\/humans. Although reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) detected <jats:italic>hlcart<\/jats:italic>\u2010transcript in the brain, CART\u2010containing neurons\/fibers were widely distributed in the telencephalon, diencephalon, mesencephalon, rhombencephalon, spinal cord, and retina. The mitral cells in olfactory bulb, neurons in the paraventricular, periventricular, arcuate (Arc), Edinger\u2013Westphal, and brainstem nuclei were intensely CART\u2010positive. In view of antagonistic roles of neuropeptide Y (NPY) and CART in energy balance in the framework of mammalian hypothalamus, we probed CART\u2013NPY interaction in the hypothalamus of <jats:italic>H. leschenaultii<\/jats:italic>. Double immunofluorescence showed a dense NPY\u2010innervation of Arc CART neurons. <jats:italic>Ex vivo<\/jats:italic> hypothalamic slices treated with NPY\/NPY\u2010Y<jats:sub>1<\/jats:sub>\u2010receptor agonist significantly reduced <jats:italic>hlcart<\/jats:italic>\u2010mRNA levels in the Arc\u2010containing tissues and CART\u2010ir in the dorsal\u2010Arc. However, CART\u2010ir in ventral\u2010Arc was unaffected. NPY via Y<jats:sub>1<\/jats:sub>\u2010receptors may regulate energy balance by inhibiting dArc CART neurons. This study on CART in a reptilian brain fills the current void in literature and underscores the conserved feature of the neuropeptide across the entire vertebrate phyla.<\/jats:p>","DOI":"10.1002\/cne.25672","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T06:25:56Z","timestamp":1728455156000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cocaine\u2010 and Amphetamine\u2010Regulated Transcript Peptide in the Central Nervous System of the Gecko, <i>Hemidactylus leschenaultii<\/i>: Molecular Characterization, Neuroanatomical Organization, and Regulation by Neuropeptide Y"],"prefix":"10.1002","volume":"532","author":[{"given":"Omprakash","family":"Singh","sequence":"first","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2010Bhubaneswar  Jatni Odisha India"},{"name":"Homi Bhabha National Institute Training School Complex  Mumbai Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumela","family":"Basu","sequence":"additional","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2010Bhubaneswar  Jatni Odisha India"},{"name":"Homi Bhabha National Institute Training School Complex  Mumbai Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhinav","family":"Srivastava","sequence":"additional","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2010Bhubaneswar  Jatni Odisha India"},{"name":"Homi Bhabha National Institute Training School Complex  Mumbai Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipti R.","family":"Pradhan","sequence":"additional","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2010Bhubaneswar  Jatni Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pallabi","family":"Dandapat","sequence":"additional","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2010Bhubaneswar  Jatni Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandramohan","family":"Bathrachalam","sequence":"additional","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2010Bhubaneswar  Jatni Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3958-0204","authenticated-orcid":false,"given":"Praful S.","family":"Singru","sequence":"additional","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2010Bhubaneswar  Jatni Odisha India"},{"name":"Homi Bhabha National Institute Training School Complex  Mumbai Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23532"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2014.74"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-39063-y"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15479"},{"key":"e_1_2_10_6_1","first-page":"1021","article-title":"Multiple Behavioral Effects of Cocaine\u2010 and Amphetamine\u2010Regulated Transcript (CART) Peptides in Mice: CART 42\u201389 and CART 49\u201389 Differ in Potency and Activity","volume":"299","author":"Bannon A. 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