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Scgn is widely expressed in the mammalian brain and has emerged as a novel neuroendocrine regulator. Although scgn is considered as a phylogenetically conserved CBP, little is known about its significance in the brain of nonmammalian vertebrates. Herein, we characterized the scgn\u2010encoding cDNA and mapped scgn\u2010equipped elements in the brain and spinal cord of a gecko,\n                    <jats:italic>Hemidactylus leschenaultii<\/jats:italic>\n                    (\n                    <jats:italic>hl<\/jats:italic>\n                    ). In the brain, a 1303\u2010bp\n                    <jats:italic>hlscgn<\/jats:italic>\n                    transcript containing 822\u2010bp cds and 481\u2010bp 3\u2032 untranslated region (UTR) was identified. The\n                    <jats:italic>hlscgn<\/jats:italic>\n                    transcript predicted to encode a hexa EF\u2010hand 273\u2010aa hlscgn showed 70%\u201390% similarity with scgn from other vertebrates. The EF2\u2010motif in hlscgn showed highest Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010binding affinity as compared to that in scgn from other vertebrates. The anti\u2010scgn antiserum detected a \u223c32\u2010kDa band in the brain, and cell bodies\/fibers in the cortex, dorsal ventricular ridge, amygdalae, striatum\/septum, hypothalamic\/thalamic nuclei, substantia nigra, interpeduncular, solitary tract, and raphe nuclei, and spinal cord. The scgn neurons in the paraventricular (PVN) and supraoptic nuclei co\u2010expressed either thyrotropin\u2010releasing hormone or vasotocin, but not mesotocin. As insulin regulates the activity of PVN neurons in mammals, and scgn expression in the mammalian\/teleost brains, the superfused hypothalamic slices of\n                    <jats:italic>H. leschenaultii<\/jats:italic>\n                    were treated with insulin, and the changes in scgn\u2010ir\/\n                    <jats:italic>hlscgn<\/jats:italic>\n                    \u2010mRNA in PVN were analyzed. Compared to control, insulin treatment significantly increased scgn expression. Thus, scgn seems to be a highly conserved protein in the vertebrate hierarchy with a variety of attributes, inclusive of neuroanatomy, neuroendocrine control, and regulation by insulin.\n                  <\/jats:p>","DOI":"10.1002\/cne.70094","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:06:30Z","timestamp":1761015990000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Secretagogin in the Central Nervous System of the Gecko,\n                    <i>Hemidactylus leschenaultii<\/i>"],"prefix":"10.1002","volume":"533","author":[{"given":"Abhinav","family":"Srivastava","sequence":"first","affiliation":[{"name":"School of Biological Sciences National Institute of Science Education and Research (NISER)\u2014Bhubaneswar  Jatni Odisha India"},{"name":"Homi Bhabha National Institute, Training School Complex  Mumbai 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