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Transformation was effected to establish permanent lines of these particular adult brain cells so that sufficient quantities would be available for other research projects. The procedure previously used to transform human fibroblasts (Koprowski et al., '62) was successfully applied to human brain cells. The success of transformation was dependent on the growth condition of the cultures at the time of infection. Events occurring after viral infection and during the pre\u2010transformation and the post\u2010transformation phases are described.<\/jats:p>","DOI":"10.1002\/cne.901610304","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T16:05:54Z","timestamp":1104595554000},"page":"317-328","source":"Crossref","is-referenced-by-count":20,"title":["Human brain in tissue culture. III. PML\u2010SV40\u2010induced transformation of brain cells and establishment of permanent lines"],"prefix":"10.1002","volume":"161","author":[{"given":"Daniela","family":"Santoli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zofia","family":"Wroblewska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald H.","family":"Gilden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Girardi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hilary","family":"Koprowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(73)90470-9"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","unstructured":"Duch D. 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