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In whole mounts, smooth fluorescent fibers were seen in a fine\u2010meshed plexus from the sphincter margin to the ciliary processes. Superimposed, a sparse pattern of thick meandering axon bundles were seen. Electroblotting and peroxidase immunochemical staining techniques unequivocally showed the presence of all three NF polypeptides in the adult rat iris.<\/jats:p><jats:p>Adult mouse irides showed a somewhat sparser pattern of NF\u2010positive nerves than that of the rat. Adult guinea pig irides contained irregular NF\u2010positive fibers and few axon bundles. In cryostat\u2010sectioned cat iris numerous irregularly distributed individual fibers were found, whereas in similarly sectioned cow iris thick NF\u2010positive axon bundles were more numerous. By embryonic day 18 numerous sparse NF\u2010positive axons were seen, and the subsequent gradual increase in both axons in bundles and fine\u2010meshed plexuses of individual fibers produced an appearance similar to that in the adult by 6 days of postnatal age. One week after grafting of irides to the anterior eye chamber, most NF\u2010positive nerves had disappeared from the iris grafts. Sympathetic and parasympathetic denervation of the irides did not influence the distribution of the NF\u2010positive iris nerves. Five days after electrothermal lesion of the trigeminal nerve just distal to its ganglion a large proportion of the NF\u2010positive nerves had disappeared from the iris. All perikarya in the parasympathetic ciliary and most perikarya in the superior cervical sympathetic and in the trigeminal sensory ganglion showed NF immunoreactivity.<\/jats:p><jats:p>The present report shows a way to visualize nonautonomic nerve populations in stretch\u2010prepared as well as sectioned irides by immunofluorescence techniques using an antiserum to neurofilament protein.<\/jats:p>","DOI":"10.1002\/cne.902230310","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T20:40:40Z","timestamp":1117226440000},"page":"457-470","source":"Crossref","is-referenced-by-count":45,"title":["Appearance and distribution of neurofilament immunoreactivity in iris nerves"],"prefix":"10.1002","volume":"223","author":[{"given":"\u00c5ke","family":"Seiger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doris","family":"Dahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"Ayer\u2010LeLievre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e5kan","family":"Bj\u00f6rklund","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.900390103"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/298084a0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1981.tb04676.x"},{"key":"e_1_2_1_5_1","unstructured":"Ayer\u2010LeLievre C. 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