{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T16:54:23Z","timestamp":1746809663300,"version":"3.40.5"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":7087,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1985,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The location of neurons that concentrate progestin in the brains of female cynomolgus monkeys was mapped by autoradiography using the specific synthetic progestin receptor ligand <jats:sup>3<\/jats:sup>H\u2010ORG 2058. Three females were ovariectomized and treated with estrogen (20 \u03bcg estradiol benzoate daily for 7 days), and one of them was also pretreated with progesterone. Each received an i.v. injection of 1 mCi <jats:sup>3<\/jats:sup>H\u2010ORG 2058 and was killed 1 hour later. Thaw\u2010mount autoradiograms revealed intense accumulation of radioactivity in the nuclei of many neurons in the mediobasal hypothalamus, particularly in the ventromedial nucleus (n.), arcuate n., and premammillary n. Neuronal labeling was also observed frequently in the medial preoptic n., and occasionally in the anterior hypothalamic area, paraventricular n., and organum vasculosum of the lamina terminalis. In the pituitary gland, about 5% of cells in the pars distalis were intensely labeled. In the female pretreated with progesterone, however, labeling was almost completely blocked. Analysis of samples by high\u2010performance liquid chromatography demonstrated that the radioactivity extracted from brain and pituitary gland cell nuclei was almost entirely unmetabolized <jats:sup>3<\/jats:sup>H\u2010ORG 2058. The nuclear concentration of progestin was much greater in the pituitary gland than in the brain, and was greater in the hypothalamus than in any other brain area. These results revealed well\u2010localized groups of progestin\u2010concentrating neurons in the primate brain which presumably mediate the effects of progresterone on both gonadotropin secretion and female sexual behavior.<\/jats:p>","DOI":"10.1002\/cne.902350305","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T19:31:09Z","timestamp":1104607869000},"page":"336-342","source":"Crossref","is-referenced-by-count":15,"title":["Localization of the synthetic progestin <sup>3<\/sup>H\u2010ORG 2058 in neurons of the primate brain: Evidence for the site of action of progestins on behavior"],"prefix":"10.1002","volume":"235","author":[{"given":"Howard D.","family":"Rees","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert W.","family":"Bonsall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard P.","family":"Michael","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(77)90064-6"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(83)90254-0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0620315"},{"key":"e_1_2_1_5_1","first-page":"13","volume-title":"The Hypothalamus","author":"Christ J. 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