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ChAT is the synthetic enzyme for acetylcholine found in neurons using that neurotransmitter; thus ChAT\u2010IR may be interpreted as indicating putative cholinergic activity. Robust staining is seen in all cranial nerve motor nuclei. In addition, the brainstem of <jats:italic>Porichthys<\/jats:italic> is distinguished by two other expansive ChAT\u2010IR zones: a sonic motor nucleus, which innervates swimbladder \u201cdrum\u201d muscles, and an octavolateralis efferent nucleus, which innervates acoustic, vestibular, and lateral line end organs. Scattered labeled cells are found in several cranial sensory nuclei\u2013the vagal lobe, and the main and descending trigeminal nuclei. ChAT\u2010IR cells form restricted subpopulations in other noncranial nerve nuclei, including the granule cell layer of the cerebellum; superior, medial, and inferior divisions of the reticular formation; the stratum periventriculare of the midbrain's optic tectum; and the nucleus isthmi in the midbrain tegmentum. In the telencephalon, a dense population of ChAT\u2010IR cells is found in the ventral nucleus of area ventralis; terminals and fine fibers are found in the dorsal, medial, and central nuclei of area dorsalis. Together, the data represent the first complete report of ChAT\u2010IR cell bodies in the brain of any nonmammal with the monoclonal antibody Ab8, which has already been extensively used on a variety of vertebrate brains. The results are thus discussed from a comparative viewpoint, considering reports of ChAT\u2010IR in different taxa.<\/jats:p>","DOI":"10.1002\/cne.902750108","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T18:56:57Z","timestamp":1104605817000},"page":"87-105","source":"Crossref","is-referenced-by-count":99,"title":["Cholinergic neurons in the brain of a teleost fish (<i>Porichthys notatus<\/i>) located with a monoclonal antibody to choline acetyltransferase"],"prefix":"10.1002","volume":"275","author":[{"given":"Richard K.","family":"Brantley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew H.","family":"Bass","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/32.8.6379037"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902160106"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051690107"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000121631"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051740307"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000118725"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000118688"},{"key":"e_1_2_1_9_1","first-page":"399","article-title":"A sexually dimorphic sonic (acoustic) motor system in a teleost fish: Peripheral and central elements","volume":"13","author":"Bass A. 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