{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T08:25:20Z","timestamp":1778574320271,"version":"3.51.4"},"reference-count":68,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":5084,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1990,11,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An immunocytochemical peroxidase\u2010antiperoxidase procedure using a purified polyclonal antibody raised against human placental aromatase was used to localize aromatase\u2010containing cells in the brain of three avian species: the Japanese quail, the ring dove, and the zebra finch. In quail and dove, immunoreactive cells were found only in the preoptic area and hypothalamus, with a high density of positive cells being present in the medial preoptic area, in the septal area above the anterior commissure, in the ventromedial nucleus of the hypothalamus, and in rostral part of the infundibulum. Immunoreactivity was weaker in zebra finches, and no signal could therefore be detected in the ventromedial and tuberal hypothalamus. The positive material was localized in the perikarya and in adjacent cytoplasmic processes, including the full length of axons always leaving a clear unstained cell nucleus. These features could be observed in more detail on sections cut from perfused brains and stained with an alkaline phosphatase procedure. The distribution of aromatase immunoreactivity was similar in the three species although minor differences were observed in the preoptic area. The localization of labelled neurons coincided with the distribution of aromatase activity as studied by in vitro radioenzyme assays on brain nuclei dissected by the Palkovits punch method. There was one striking exception to this rule: no immunoreactivity was detected in the zebra finch telencephalon, while assays had shown the presence of an active enzyme in several nuclei such as the robustus archistriatalis, the hyperstriatum ventrale pars caudale, and the hippocampus and area parahippocampalis. The origins of this discrepancy and the functional role of the aromatase observed in the axons are discussed.<\/jats:p>","DOI":"10.1002\/cne.903010210","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T19:39:37Z","timestamp":1104608377000},"page":"276-288","source":"Crossref","is-referenced-by-count":166,"title":["Distribution of aromatase in the brain of the Japanese quail, ring dove, and zebra finch: An immunocytochemical study"],"prefix":"10.1002","volume":"301","author":[{"given":"Jacques","family":"Balthazart","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnes","family":"Foidart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantal","family":"Surlemont","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Vockel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhiro","family":"Harada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(80)90233-4"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(81)90125-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(86)90429-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/13813458909075078"},{"key":"e_1_2_1_6_1","first-page":"1188","article-title":"The induction of aromatase and sexual behavior by testosterone in male and female Japanese quail: A dose\u2010response study. I","volume":"14","author":"Balthazart J.","year":"1986","journal-title":"R.C.S. Med. Sci."},{"key":"e_1_2_1_7_1","first-page":"31","article-title":"Plasma levels of luteinizing hormone and of five steroids in photostimulated, castrated and testosterone\u2010treated male and female Japanese quail","volume":"5","author":"Balthazart J.","year":"1987","journal-title":"Coturnix japonica'. General Endocrinol. (Life Sci. Adv."},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)90638-0"},{"key":"e_1_2_1_9_1","first-page":"18","article-title":"Immunocytochemical localization of aromatase in the quail brain","volume":"430","author":"Balthazart J.","year":"1989","journal-title":"Soc. Neurosci. Meeting AB"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(90)90045-6"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)91428-J"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","unstructured":"Balthazart J. M.Schumacher andL.Evrard(1990c) Sex differences and steroid control of testosterone\u2010metabolizing enzymes activity in the quail brain.J. Neuroendocrinol.(in press).","DOI":"10.1111\/j.1365-2826.1990.tb00464.x"},{"key":"e_1_2_1_13_1","volume-title":"Hormones, Brain and Behavior in Vertebrates. Comparative Physiology","author":"Balthazart J.","year":"1990"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","unstructured":"Balthazart J. L.Evrard andC.Surlemont(1990e) Effects of the non\u2010steroidal aromatase inhibitor R76713 on testosterone\u2010induced sexual behavior in the Japanese quail (Coturnix coturnix japonica).Horm. Behav.(in press).","DOI":"10.1016\/0018-506X(90)90039-Z"},{"key":"e_1_2_1_15_1","first-page":"219","article-title":"Stereotaxic topography of the brain of the quail","volume":"68","author":"Bayl\u00e9 J. D.","year":"1974","journal-title":"J. Physiol. (Paris)"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(87)90107-9"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902030305"},{"key":"e_1_2_1_18_1","first-page":"31","volume-title":"Advances in Comparative and Environmental Physiology","author":"Blaustein J. D.","year":"1989"},{"key":"e_1_2_1_19_1","first-page":"61","volume-title":"The Hypothalamus","author":"Crosby E. C.","year":"1969"},{"key":"e_1_2_1_20_1","first-page":"8","article-title":"Inhibition of testosterone\u2010induced copulation in quail by a novel specific aromatase inhibitor, R76713","volume":"155","author":"Evrard L.","year":"1989","journal-title":"Soc. Neurosci. Meeting AB"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-65-4-757"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(87)91063-8"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.19.7380"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(84)90188-2"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a122213"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(83)90021-1"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.900480102"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-6-2180"},{"key":"e_1_2_1_29_1","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1159\/000124558","article-title":"Formation of behaviorally active estrogen in the dove brain: Induction of preoptic aromatase by intracranial testosterone","volume":"43","author":"Hutchison J. B.","year":"1986","journal-title":"Neuroendocrinology"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(89)90041-X"},{"key":"e_1_2_1_31_1","first-page":"18","article-title":"Brain areas forming behaviorally effective estrogens by aromatase activity contain immunoreactive estrogen receptors","volume":"155","author":"Hutchison J. B.","year":"1989","journal-title":"Soc. Neurosci. Meeting AB"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod41.5.889"},{"key":"e_1_2_1_33_1","volume-title":"A stereotaxic atlas of the brain of the pigeon (Columba livia)","author":"Karten H. J.","year":"1967"},{"key":"e_1_2_1_34_1","first-page":"225","volume-title":"Biological Determinants of Sexual Behaviour","author":"Kelley D. B.","year":"1978"},{"key":"e_1_2_1_35_1","volume-title":"A Stereotaxic Atlas of the Brain of the Chick (Gallus domesticus)","author":"Kuenzel W. J.","year":"1988"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902060309"},{"key":"e_1_2_1_37_1","first-page":"166","volume-title":"Anatomical Neuroendocrinology","author":"Martinez\u2010Vargas M. C.","year":"1975"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901670106"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(85)90525-9"},{"key":"e_1_2_1_40_1","first-page":"295","article-title":"The formation of estrogens by central neuroendocrine tissues","volume":"32","author":"Naftolin F.","year":"1975","journal-title":"Rec. Prog. Horm. Res."},{"key":"e_1_2_1_41_1","unstructured":"Naftolin F. C.Leranth andJ.Balthazart(1990) Ultrastructural localization of aromatase immunoreactivity in hypothalamic neurons.Endocrine Soc. Abstr.(Abstract)."},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.480180607"},{"key":"e_1_2_1_43_1","first-page":"1","article-title":"Neurohistological studies of the hypothalamo\u2010hypophysial system of Zonotrichia leucrophrys gambelii (Aves, Passeriformes) with special attention to its role in the control of reproduction","volume":"48","author":"Oksche A.","year":"1974","journal-title":"Adv. Anat. Embryol. Cell Biol."},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(73)90290-4"},{"key":"e_1_2_1_45_1","first-page":"1","volume-title":"Brain Microdissection Techniques","author":"Palkovits M.","year":"1983"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(87)91496-X"},{"key":"e_1_2_1_47_1","doi-asserted-by":"crossref","unstructured":"Panzica G. C. C.Viglietti\u2010Panzica F.Sanchez Ph.Sante andJ.Balthazart(1990) Effects of testosterone on a selected neuronal population within the preoptic sexually dimorphic nucleus of the Japanese quail.J. Comp. Neurol.(submitted).","DOI":"10.1002\/cne.903030310"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-117-6-2471"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19971201)388:4<507::AID-CNE1>3.0.CO;2-6"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(89)90010-5"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0850163"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1159\/000125149"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)64428-3"},{"key":"e_1_2_1_54_1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0006-8993(86)90483-X","article-title":"Testosterone\u2010induced brain aromatase is sexually dimorphic","volume":"370","author":"Schumacher M.","year":"1986","journal-title":"Brain Res."},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(87)90548-8"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902840304"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902900405"},{"key":"e_1_2_1_58_1","first-page":"20","article-title":"Aromatase neurons in the monkey forebrain demonstrated by antibody against human placental antigen X\u2010P2","volume":"96","author":"Shinoda K.","year":"1989","journal-title":"Soc. Neurosci. Meeting AB"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/292345a0"},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)90203-5"},{"key":"e_1_2_1_61_1","unstructured":"Steimer Th.(1988) Aromatase activity in rat brain synaptosomes. Is an enzyme associated with the neuronal cell membrane involved in mediating non\u2010genomic effects of androgens.Eur. J. Neurosci.Suppl 9."},{"key":"e_1_2_1_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(80)90912-9"},{"key":"e_1_2_1_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901560305"},{"key":"e_1_2_1_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(86)90087-X"},{"key":"e_1_2_1_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90174-A"},{"key":"e_1_2_1_66_1","doi-asserted-by":"crossref","unstructured":"Vockel A. E.Pr\u00f6ve andJ.Balthazart(1990b) Effects of castration and testosterone treatment on the activity of testosterone metabolizing enzymes in the brain of male and female zebra finches.J. Neurobiol.(in press).","DOI":"10.1002\/neu.480210514"},{"key":"e_1_2_1_67_1","first-page":"351","volume-title":"Neural and Endocrine Aspects of Behaviour in Birds","author":"Vowles D. M.","year":"1975"},{"key":"e_1_2_1_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/0018-506X(89)90055-X"},{"key":"e_1_2_1_69_1","doi-asserted-by":"publisher","DOI":"10.1159\/000125091"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.903010210","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.903010210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T15:40:47Z","timestamp":1697989247000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.903010210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,11,8]]},"references-count":68,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,11,8]]}},"alternative-id":["10.1002\/cne.903010210"],"URL":"https:\/\/doi.org\/10.1002\/cne.903010210","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,11,8]]}}}